Planta Med 2019; 85(18): 1481
DOI: 10.1055/s-0039-3399877
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Discovery of novel skin-whitening agents produced by endophytic fungi associated with desert plants. The case of Comoclathris spp

K Georgousaki
1   Fundacion MEDINA, Avda., del Conocimiento, 34, 18016, Armilla, Granada, Spain
2   National and Kapodistrian University of Athens,, Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Panepistimiopolis, 15771, Athens, Greece
,
V González-Menéndez
1   Fundacion MEDINA, Avda., del Conocimiento, 34, 18016, Armilla, Granada, Spain
,
JR Tormo
1   Fundacion MEDINA, Avda., del Conocimiento, 34, 18016, Armilla, Granada, Spain
,
N Tsafantakis
2   National and Kapodistrian University of Athens,, Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Panepistimiopolis, 15771, Athens, Greece
,
TA Mackenzie
1   Fundacion MEDINA, Avda., del Conocimiento, 34, 18016, Armilla, Granada, Spain
,
F Reyes
1   Fundacion MEDINA, Avda., del Conocimiento, 34, 18016, Armilla, Granada, Spain
,
J Martín
1   Fundacion MEDINA, Avda., del Conocimiento, 34, 18016, Armilla, Granada, Spain
,
N Fokialakis
2   National and Kapodistrian University of Athens,, Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Panepistimiopolis, 15771, Athens, Greece
,
O Genilloud
1   Fundacion MEDINA, Avda., del Conocimiento, 34, 18016, Armilla, Granada, Spain
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Endophytic fungi have been identified as a significant source of novel natural bioactive products with potential application in several fields as agriculture, medicine and food industry [1], [2], [3]. In order to explore the potential of endophytic fungi to produce secondary metabolites with applications in the cosmeceutical industry, 142 diverse endophytes from a wide variety of Spanish arid plants were selected to be screened for their skin-whitening activity, applying the OSMAC approach. Cytotoxicity evaluation was performed to discriminate cytotoxic fungal extracts [4]. Two fungal strains CF-090361 and CF-090766, isolated from Sedum sediforme and Nerium oleander, were selected as the most promising. Phylogenetic analyses support that both strains could represent a new species of Comoclathris. The aim of this study was to assess the effect of different fermentation conditions on the tyrosinase inhibitory activity and to isolate and identify the active secondary metabolites. Tyrosinase bio-guided assay of both strains led to the isolation of the main active components. Two known compounds Brevianamide M (IC50= 6.81 μΜ), Graphostrin B (IC50=86.32 μΜ), and three new compounds with molecular formula C12H12O3 (IC50=3.5 μΜ), C11H16O3 (IC50=853.72 μΜ) and C12H10O4 (IC50=0.16 μΜ) were identified as the main responsible for the whitening activity.

Volcano-plots based on LCMS were applied to determine significant metabolomic differences between both strains and the different fermentation conditions tested, highlighting these bioactive secondary metabolites. Overall, these results can serve as proof of concept that endophytic fungi can be a source of bioactive secondary metabolites that can potentially be used in the cosmeceutical industry.

Acknowledgements This work has been financially supported by Fundación MEDINA, the Hellenic Foundation for Research and Innovation (HFRI) and the General Secretariat for Research and Technology (GSRT), under the HFRI PhD Fellowship grant (GA. no. 2369).

 

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