Planta Med 2019; 85(18): 1478-1479
DOI: 10.1055/s-0039-3399871
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Chilean “chaura” berries (Gaultheria phillyreifolia and G. poeppigii): isolation of secondary metabolites and antioxidant activity

D Mieres-Castro
1   Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca,, Talca, Chile
,
G Schmeda-Hirschmann
1   Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca,, Talca, Chile
,
C Theoduloz
2   Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca,, Talca, Chile
,
S Gómez-Alonso
3   Instituto Regional de Investigación Científica Aplicada, Universidad de Castilla-La Mancha,, Ciudad Real, España
,
J Pérez-Navarro
3   Instituto Regional de Investigación Científica Aplicada, Universidad de Castilla-La Mancha,, Ciudad Real, España
,
K Márquez
4   Biotechnology Centre and Chemical Sciences Faculty, University of Concepción,, Concepción, Chile
,
F Jiménez-Aspee
5   Departamento de Ciencias Básicas Biomédicas, Facultad de Ciencias de la Salud, Universidad de Talca,, Talca, Chile
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Berries from the Gaultheria genus (Ericaceae) are used worldwide as food and in traditional medicine [1]. Most studies have been carried out with Asian [2], North American and European species [3], [4]. Five edible Gaultheria species occurs in Chile [5] and little is known on their secondary metabolite chemistry. The aims of the present work were to isolate the main secondary metabolites from Gaultheria phillyreifolia and G. poeppigii berries, and to evaluate their antioxidant activity. A combination of membrane chromatography (MC) and counter-current chromatography (CCC) was used to isolate the main compounds. The products were characterized by spectroscopic and spectrometric means. The antioxidant activity was measured by different spectrophotometric assays. The fruit extracts were submitted to MC to separate anthocyanins from copigments. Four anthocyanins were isolated by CCC and identified as galactoside and arabinoside of cyanidin and delphinidin. From the copigment fraction, CCC allowed the isolation of quercetin-3-arabinoside, 3-rutinoside and 3-rhamnoside, 3-caffeoylquinic acid and the iridoids monotropein-10-trans-coumarate, monotropein-10-trans-cinnamate and 6α-hydroxy-dihydromonotropein-10-trans-cinnamate. The latter two compounds are new natural products. Other 34 compounds were tentatively identified by HPLC-DAD-ESI-MSn. The G. phillyreifolia samples showed better antioxidant activity in the FRAP, TEAC and CUPRAC assays. On the other hand, the G. poeppigii samples showed better results in the DPPH and ORAC assays. The observed differences between both species were validated by a Partial Least Square Discriminant Analysis (PLS-DA). This is the first report on the isolation and characterization of secondary metabolites from Chilean Gaultheria phillyreifolia and G. poeppigii berries.

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Fig. 1
 
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