Planta Med 2019; 85(18): 1474
DOI: 10.1055/s-0039-3399860
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Bio-guided phytochemical study of Plectranthus mutabilis Codd.

E Ntungwe
1   CBIOS – Center Research for Biosciences & Health Technologies, Universidade Lusófona de Humanidades e Tecnologias,, Campo Grande 376, 1749-024 Lisbon, Portugal
2   Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcalá,, Ctra. A2, Km 33.600 – Campus Universitario, 28871 Alcalá de Henares, Spain
,
V Isca
1   CBIOS – Center Research for Biosciences & Health Technologies, Universidade Lusófona de Humanidades e Tecnologias,, Campo Grande 376, 1749-024 Lisbon, Portugal
3   Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia da Universidade de Lisboa,, Av. Prof. Gama Pinto 1649-003 Lisbon, Portugal
,
M Vágvölgyie
4   Institute of Pharmacognosy, University of Szeged,, Eötvös str. 6. 6720 Szeged, Hungary
,
G Spengler
5   Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged,, H-6720 Szeged, Hungary
,
A Hunyadi
4   Institute of Pharmacognosy, University of Szeged,, Eötvös str. 6. 6720 Szeged, Hungary
,
P Rijo
1   CBIOS – Center Research for Biosciences & Health Technologies, Universidade Lusófona de Humanidades e Tecnologias,, Campo Grande 376, 1749-024 Lisbon, Portugal
3   Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia da Universidade de Lisboa,, Av. Prof. Gama Pinto 1649-003 Lisbon, Portugal
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Natural products from medicinal plants represent major resource of novel therapeutic substances for combating serious diseases including cancer and infections. The Plectranthus genus (Family: Lamiaceae) represents a large and widespread group of species with a diversity of traditional uses for the treatment of several ailments [1]. P. mutabilis Codd. chemical constituents have been cited on its HPLC analysis that revealed the presence of Nepetoidins A and Nepetoidins B [2]. We therefore aimed to study the composition and biological activity of this plant to reinforce the low phytochemical information. In this study, the air-dried P. mutabilis whole plant was extracted in acetone using the ultra-sound assisted extraction method. Furthermore, a bio-guided fractionation was performed followed by an Artemia salina general toxicity assay, DPPH-antioxidant activity and antimicrobial activity [3]. The extract was subjected to different column chromatographies using silica or polyamide with increasing polarity to afford the metabolite coleon U (1). The fully structure characterization was done mainly by 1D- and 2D-NMR and comparison with literature data. Compound 1 was tested against two Gram positive (Minimum Inhibitory Concentration - MIC values: Staphylococcus aureus = 1.56 µg/mL, Enterococcus faecalis = 25 µg/mL) and two Gram negative bacteria (Klebsiella pneumoniae = >100 µg/mL, Escherichia coli = >100 µg/mL). Additionally, the isolated diterpenoid showed moderate cytotoxicity in Human cancer and normal cell lines (Colo 205, multidrug resistant overexpressing ABCB1 Colo 320 and MRC-5) showing slight selectivity to resistant cancer cells. Further phytochemical studies are ongoing.

Zoom Image
Fig. 1 Coleon U (1)
 

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