Planta Med 2019; 85(18): 1466
DOI: 10.1055/s-0039-3399840
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

NMR metabolic profiling of Greek Pistacia lentiscus leaves and fruit extracts for the identification of biomarkers with skin beneficial effects

A Cheilari
1   Department of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens,, Panepistimioupolis Zografou, 15771, Athens, Greece
,
P Papalexis
1   Department of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens,, Panepistimioupolis Zografou, 15771, Athens, Greece
,
A Vontzalidou
1   Department of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens,, Panepistimioupolis Zografou, 15771, Athens, Greece
,
E Dina
1   Department of Pharmacognosy and Natural Products Chemistry, National and Kapodistrian University of Athens,, Panepistimioupolis Zografou, 15771, Athens, Greece
,
I Smyrnioudis
2   Chios Mastic Gum Growers Association,, 1 K. Monomachou St., Chios, GR 82100, Chios, Greece
,
N Aligiannis
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

The healing properties of Mastic resin are well-known in the Mediterranean basin for more than 2500 years. Nowadays, the resin, a PDO product, has been well-studied and showed antimicrobial, anti-inflammatory, and other activities [1]. However, the fruit and leaves of Pistacia lentiscus L. shrub from Chios have not been thoroughly investigated and additionally, they are discarded as considered by-products of the mastic tree cultivation.

In continuation of our research [2], this study emphasises on the exploration of the biological properties of P. lentiscus leaves and fruit collected from the region of Mastichochoria. Leaves (36 samples) and fruit (89 samples) were collected from different altitudes and distance from the sea [Fig. 1] during the maturing period. All samples were extracted with various NMR solvents and their NMR metabolic profiling spectra were acquired. In parallel, their antioxidant capacity as well as their enzyme inhibition properties (tyrosinase, elastase, collagenase, hyaluronidase) related to skin whitening, moisture and elasticity were evaluated. Finally, NMR spectral data were processed with SIMCA software (Umetrics) and multivariate statistical analysis (PCA, OPLS-DA) was used to evaluate the quality characteristics of the samples and to identify potential biomarkers. This approach resulted in the detection of more than 40 spectral features (biomarkers) in all of the extracts and facilitated the classification of samples. To the best of our knowledge, this research effort is the first to study the NMR-based chemical profiling of P. lentiscus leaves and fruit from different regions of Chios and correlate their enzyme inhibition activity by Multivariate Data Analysis.

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Fig. 1
 

  • References

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