Planta Med 2019; 85(18): 1463
DOI: 10.1055/s-0039-3399834
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Investigation of Greek honeys using HR-NMR and LC-HRMS metabolomics, for determination of their geographical, botanical origin and authenticity

GB Lemus Ringele
1   Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens,, Panepistimiopolis, Zografou 15771, Athens, Greece
,
E Axiotis
1   Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens,, Panepistimiopolis, Zografou 15771, Athens, Greece
,
S Katsikis
1   Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens,, Panepistimiopolis, Zografou 15771, Athens, Greece
,
A Argyropoulou
1   Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens,, Panepistimiopolis, Zografou 15771, Athens, Greece
,
LA Skaltsounis
1   Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens,, Panepistimiopolis, Zografou 15771, Athens, Greece
,
M Halabalaki
1   Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens,, Panepistimiopolis, Zografou 15771, Athens, Greece
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Honey is a natural source of nutrition used since antiquity. Nowadays, it is well known that can be also applied, among others, in cosmetics, food industry and environmental engineering [1],[2]. However, due to its high economic importance authenticity and adulteration are major issues mainly towards geographical and botanical origin. It should be noted that the sugars have been mainly studied, though limited studies are focused on other polar metabolites, especially phenols, which are related not only with geographical but mainly with botanical origin. Many methodologies based on liquid chromatography, and lately, NMR have been used towards this scope. The aim of this study was to analyze Greek honeys and draw conclusions about their authenticity. Special attention was given to their content in phenolic compounds. Honeys were collected from different islands of the northern east Aegean Sea in Greece (Agios Efstratios, Ikaria, Lesbos, Lemnos, Samos, Fournoi, Chios and Psara), with different herbal origin. Extraction procedure previously reported [3] was carried out with slight modifications. The profiles of the extracts were obtained with both LC-HRMS and NMR. Dereplication based on high resolution mass spectra gave information about the composition of the different honeys, while in NMR profiling in combination with multivariate data analysis enabled the classification of samples. Moreover, significant markers responsible for the observed clusters were identified. Finally, statistical data correlations from both platforms are in progress.

 

  • References

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