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CC BY-NC-ND 4.0 · Organic Materials 2019; 01(01): 038-042
DOI: 10.1055/s-0039-1700849
Short Communication
The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/). (2019) The Author(s).

Application of Cationic Conjugated Polymer–Outer Membrane Vesicle Complexes in Inhibiting Red Blood Cell Aggregation

Ping He
a   Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
,
Endong Zhang
a   Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
b   School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, China
,
Ruilian Qi
a   Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
,
Fengting Lv
a   Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
,
Libing Liu 
a   Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
b   School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, China
,
Shu Wang 
a   Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
b   School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, China
› Institutsangaben Funding Information: The authors would like to thank the National Natural Science Foundation of China (21773268, 21533012, and 21661132006) for financial support.
Weitere Informationen

Publikationsverlauf

Received: 06. August 2019

Accepted after revision: 16. September 2019

Publikationsdatum:
28. November 2019 (online)

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Abstract

Cationic conjugated polymers (CCPs) have been attracting a lot of attention in biomedical applications because of their good photoelectric properties and good cell viability. However, positively charged components may reduce the electrostatic repulsion among red blood cells (RBCs) and induce RBC aggregation, which may lead to thrombus. Herein, in this work, we prepared the complexes of CCPs and outer membrane vesicles (OMVs) to inhibit RBC aggregation induced by CCPs and improve the biocompatibility of CCPs. A poly(fluorene-co-phenylene) derivative (PFP), a poly(p-phenylenevinylene) derivative (PPV), and a poly(thiophene) derivative (PMNT) were chosen as the model CCPs, and OMVs were used as the representative of a cell membrane. The CCP–OMV complexes were formed mainly driven by electrostatic interactions. Besides, the electrostatic CCP–OMV complexes were proved to be able to prevent the RBC aggregation induced by CCPs while maintaining the hydrophobic interactions between CCPs and RBCs.

Key words

cationic conjugated polymers - outer membrane vesicles - inhibition of RBC aggregation

Supporting Information

Supporting information for this article is available online at https://doi.org/10.1055/s-0039-1700849.


Supporting Information

 

  • References and Notes

  • 1 Wang TT, Jiang X. ACS Appl. Mater. Interfaces 2015; 7: 129
    CrossrefPubMed
      • 2
    • 2a Han Y, Wang X, Dai H, Li S. ACS Appl. Mater. Interfaces 2012; 4: 4616
      CrossrefPubMed
    • 2b Jan KM, Chien S. J. Gen. Physiol. 1973; 61: 638
      CrossrefPubMed
    • 2c Jan KM. Bioelectrochemistry 1980; 188: 143
      PubMed
    • 2d Snabre P, Grossmann GH, Mills P. Colloid Polym. Sci. 1985; 263: 478
      CrossrefPubMed
    • 2e Armstrong JK, Meiselman HJ, Fisher TC. Am. J. Hematol. 1997; 56: 26
      CrossrefPubMed
    • 2f Toth K, Wenby RB, Meiselman HJ. Biorheology 2000; 37: 301
      PubMed
    • 2g Byrnes JR, Wolberg AS. Blood 2017; 130: 1795
      CrossrefPubMed
  • 3 Zhu CL, Liu LB, Yang Q, Lv FT, Wang S. Chem. Rev. 2012; 112: 4687
    CrossrefPubMed
      • 4
    • 4a Disney MD, Zheng J, Swager TM, Seeberger PH. J. Am. Chem. Soc. 2004; 126: 13343
      CrossrefPubMed
    • 4b Yuan HX, Liu Z, Liu LB, Lv FT, Wang YL, Wang S. Adv. Mater. 2014; 26: 4333
      CrossrefPubMed
    • 4c Zhu CL, Yang Q, Liu LB. , et al. Adv. Mater. 2011; 23: 4805
      CrossrefPubMed
      5
    • 5a Li M, Li SL, Chen H, Hu R, Liu LB, Lv FT, Wang S. ACS Appl. Mater. Interfaces 2016; 8: 42
      CrossrefPubMed
    • 5b Li M, He P, Li SL. , et al. ACS Biomater. Sci. Eng. 2018; 4: 2037
      CrossrefPubMed
      6
    • 6a Duan XR, Li ZP, He F, Wang S. J. Am. Chem. Soc. 2007; 129: 4154
      CrossrefPubMed
    • 6b Xia F, Zuo XL, Yang RQ. , et al. J. Am. Chem. Soc. 2010; 132: 1252
      CrossrefPubMed
      7
    • 7a Zhu CL, Yang Q, Liu LB, Wang S. Chem. Commun. (Camb.) 2011; 47: 5524
      CrossrefPubMed
    • 7b Wang B, Nie CY, Liu LB, Lv FT, Wang S. Chin. Chem. Lett. 2017; 28: 1975
      CrossrefPubMed
      8
    • 8a Feng GG, Mai CK, Zhan RY, Bazan GC, Liu B. J. Mater. Chem. B Mater. Biol. Med. 2015; 3: 7340
      CrossrefPubMed
    • 8b Huang Y, Pappas HC, Zhang LQ. , et al. Chem. Mater. 2017; 29: 6389
      CrossrefPubMed
      9
    • 9a Dehaini D, Fang RH, Zhang LF. Bioeng. Transl. Med. 2016; 1: 30
      CrossrefPubMed
    • 9b Kroll AV, Fang RH, Zhang LF. Bioconjug. Chem. 2017; 28: 23
      CrossrefPubMed
    • 9c Su JH, Sun HP, Meng QS. , et al. Adv. Funct. Mater. 2016; 26: 1243
      CrossrefPubMed
    • 9d Gao WW, Fang RH, Thamphiwatana S. , et al. Nano Lett. 2015; 15: 1403
      CrossrefPubMed
    • 9e Hu CMJ, Fang RH, Copp J, Luk BT, Zhang LF. Nat. Nanotechnol. 2013; 8: 336
      CrossrefPubMed
    • 9f Yang G, Liu L, Lv F, Wang S. Sci. Rep. 2013; 3: 1702
      CrossrefPubMed
  • 10 Gaylord BS, Massie MR, Feinstein SC, Bazan GC. Proc. Natl. Acad. Sci. U.S.A. 2005; 102: 34
    CrossrefPubMed
  • 11 Yuan HX, Liu Z, Liu LB, Lv FT, Wang YL, Wang S. Adv. Mater. 2014; 26: 4333-4338
    CrossrefPubMed
  • 12 Liu XF, Tang YL, Wang LH, Zhang J, Song SP, Fan CH, Wang S. Adv. Mater. 2007; 19: 1471-1474
    CrossrefPubMed
  • 13 The preparation of CCP-OMVs complex: PPV storage solution (10 mM) was added into the OMVs solution (80 μg/mL) to obtain a final concentration of PPV in 10 μM or 100 μM. Then the mixture was placed in room temperature for 4 h to obtain PPV-OMVs. PFP-OMVs and PMNT-OMVs were prepared by the same way as PPV-OMVs
    PubMed