CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2020; 55(04): 410-414
DOI: 10.1055/s-0039-1700830
Artigos Originais

Intraoperative graft decontamination during ACL reconstruction surgery[*]

Article in several languages: português | English
1  Hospital Orthomed Center, Uberlândia, MG, Brasil
Ígor Severino Macedo
1  Hospital Orthomed Center, Uberlândia, MG, Brasil
Rafael Henrique Naves Pereira
1  Hospital Orthomed Center, Uberlândia, MG, Brasil
Daniel Barros Pereira
1  Hospital Orthomed Center, Uberlândia, MG, Brasil
Dyego Vilela Luciano
1  Hospital Orthomed Center, Uberlândia, MG, Brasil
› Author Affiliations


Objective To evaluate different decontaminants for tendon grafts, proposing an antiseptic protocol for contaminated grafts.

Methods A total of 25 patients were tissue donors for the study. Each participant donated a 2.5-cm tendon sample, which was divided into 5 fragments with 5 mm each during anterior cruciate ligament (ACL) reconstruction surgery. The collected material was divided into 5 groups, totaling 125 samples. In total, four fragments of each patient were placed on the operating room floor for one minute for contamination, simulating the fall of the graft on the floor during surgery. The other fragment was immediately placed in a sterile container (group 1). One of the contaminated fragments was placed in the sterile container without being previously immersed in decontaminating solution (group 2). The remaining fragments were immersed for ten minutes in decontaminating solution: 0.5% chlorhexidine (group 3), 0.9% saline (group 4) and 0.55% ortho-phthalaldehyde (group 5), and, after this time, they were individually placed in a sterile container. The samples from the 5 groups were submitted to microbiological examination.

Results Bacteria were detected in 26% of the total samples in the microbiological tests, and in group 1 there was no growth of microorganisms. In group 2, bacterial growth was observed in 16 samples. Considering the evaluation of test groups 3, 4 and 5, the percentage of decontamination was higher than the growth of microorganisms in the respective cultures.

Conclusion The protocol suggested by the study showed that intraoperative graft decontamination is possible.

* Study developed at Hospital Orthomed Center, Uberlândia, MG, Brazil.

Publication History

Received: 28 March 2019

Accepted: 31 July 2019

Publication Date:
09 January 2020 (online)

© 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (

Sociedade Brasileira de Ortopedia e Traumatologia. Published by Thieme Revinter Publicações Ltda
Rio de Janeiro, Brazil