CC BY-NC-ND 4.0 · National Journal of Clinical Anatomy 2017; 06(03): 179-183
DOI: 10.1055/s-0039-1700748
Original Article
Society of Clinical Anatomists

A Cytogenetic Study of Chromosomal Abnormalities in first trimester abortions in different maternal age groups

Shahin Kazi
1  Associate Professor, Department of Anatomy, A.C.P.M. Medical College, Dhule, Maharashtra
,
Harsha A. Keche
2  Associate Professor of Anatomy, A.C.P.M. Medical College, Dhule, Maharashtra, India
,
Kotwaliwale› Author Affiliations
Further Information

Publication History

Publication Date:
24 November 2019 (online)

Abstract

Background : Approximately 15% of all clinically recognized pregnancies end in spontaneous abortions. Chromosomal disorders are responsible for 50% of the spontaneous abortions. Most commonly it occurs in the first trimester of pregnancy. Aim : To study the cytogenetic analysis of chorion villous tissue in 50 cases of abortion in age groups of 19-40 years. Material and Methods : Cytogenetic analysis was performed by implementing standard protocol of planting, harvesting, banding and screening. The karyotypes were prepared and observed under microscope. Statistical analysis was done by calculating the percentage of abnormal abortions in relation to maternal age. Results : It was observed that maximum abortion took place between 25-34 years of maternal age. The rate of abortion with trisomy was maximum followed by polyploidy and monosomy. It was seen that rate of abortion with monosomy decreases with increase in maternal age. Contrary to this trisomy increased with increase in maternal age. Conclusion : Cytogenetic study revealed that the rate of trisomie abortions increased with the increase in maternal age.

 
  • References

  • 1 Vanden Berg MJ, Van Maarle MC, Vanwely M, Goddijn M, et al. Genetics of early Miscarriage. Biochimica et Biophysica Acta-Molecular Basis of Disease. December 2012; 1822(12): 1951 -1959.
  • 2 Mc Nair T, Altman K. Miscarriage and recurrent pregnancy loss. In: Hurt KJ, Guile MW, Beinstock JL, Harrold et al. John’s Hopkins Manual of Gynaecology and Obstetrics. 4th edition, Lippincott William and Wilkin’s Publication Philadelphia, USA, 2012:438- 449.
  • 3 Stephenson MD, Awariane KA, Robinson WP et al. Cytogenetic analysis of miscarriage from couples with recurrent miscarriage a case control study. Human Reproduction. 2002; 17(2):446-451.
  • 4 Qin JZ, Pang LH, Li MQ, Xu J, Zhou.X et al. Risk of chromosomal abnormalities in early spontaneous abortion after assisted reproductive technology: A meta analysis. PLOS onelO October 2013;8(10): 1-11. e7593.doi:10,1371/journal, pone 0075953.
  • 5 Nikitina VT, Sazhenova EA, Tolmacheva EN, Sukhanova NN. Comparative cytogenetic analysis of spontaneous abortion in recurrent and sporadic pregnancy losses. Biomedicine Hub. 2016; 1(1): 1-11.
  • 6 Bray I, Gunnel D, Davey smith G. “Advanced paternal age” How old is too old”. Journal of Epidemiology and community Health. 2006;60(10):851-3.
  • 7 Hansen JP. Older matemalage and pregnancy outcome. A review of literature. Obstetric and Gynaecology survey. Novl986;41(ll):726-742.
  • 8 Snijder’s RJ, Noble P, Sebive N, Souka A, Niclaides KH et al. UK multicentric proj ect on assessment of risk of trisomy 21 by maternal age and fetal translucency thickness at 10-14 weeks of gestation. Fetal medicine foundation first trimester screening group: Lancet 1998; 352(9125):343-346.
  • 9 Nicolaides P, Peterson MB. Origin and mechanism of nondisjunction in human autosomal trisomies. Human Reproduction (1998); 13(2):313-319.
  • 10 Eiben B, Borgmann’s, Schube I, Hansmann J et al. A cytogenetic study directly from chorionic villi of 140 spontaneous abortion. Human genetics. 1987;77(4):137-141.
  • 11 Geisler M, Kleinbrech J. Cytogenetic and histological analysis of spontaneous abortion. Human genetics. 1978;45(3):239-251
  • 12 Hill JA. Recurrent pregnancy loss. In: Ryan KJ, Berkowitz RS, Barbieri RL, Dynaif A. Kistner’s Gynaecology and Women’s Health. 7th edition, Mosby, inc,St Louis, Missouri, 1999:396-398.
  • 13 Lauristen JG. Aeitology of spontaneous abortion -a cytogenetic epidemiological study of 288 abortuses and their parents. Acta obstetrica et Gynaecologica Scandinavical976;52:1-29.
  • 14 Ohno M, MaedoT, Matsunobu A et al, A cytogenetic study of spontaneous abortion with direct analysis of chorionic villi. Obstetrics and Gynaecology 1991;77(3):394-398.
  • 15 Minguillon C, Eiben B, Bahr-Porsch S, Vogel M, Hansmann I. The predictive value of chorionic villus tissue histology for identifying chromosomally normal and abnormal spontaneous abortion. Human genetics. 1989;82(4):373-376.
  • 16 Hassold T, Chen N, Funkhouses J, Joss T, Manuel B et al. A cytogenetic study of 1000 spontaneous abortions. Annals of human genetics. 1980;44(2): 151-163.
  • 17 Eiben B, Bartels I, Bahr-Porsch S, Borgmann S et al. Cytogeneic analysis of 750 spontaneous abortion with the direct preparation method of chorionic villi and its implications for studying genetic cause of pregnancy wastage. Am. J. Human genetics. 1990;47 (4):656-663.
  • 18 Creasy MR. A cytogenetic study of human spontaneous abortion using banding technique. Human genetics. 1976;31(2):177-196.