CC BY-NC-ND 4.0 · Indian J Plast Surg 2011; 44(01): 118-124
DOI: 10.1055/s-0039-1699490
Original Article
Association of Plastic Surgeons of India

Soluble CD163: A novel biomarker for the susceptibility to sepsis in severe burn injuries

Andrzej Piatkowski
Department of Plastic Surgery and Hand Surgery, Burn Unit of the RWTH University Hospital in Aachen, Germany
,
Gerrit Grieb
Department of Plastic Surgery and Hand Surgery, Burn Unit of the RWTH University Hospital in Aachen, Germany
,
Rittuparna Das
1  Department of Internal Medicine, Section of Infectious Disease, The Anlyan Center, 300 Cedar Street, Yale School of Medicine, New Haven, CT 06520, USA
,
Ahmet Bozkurt
Department of Plastic Surgery and Hand Surgery, Burn Unit of the RWTH University Hospital in Aachen, Germany
,
Dietmar Ulrich
Department of Plastic Surgery and Hand Surgery, Burn Unit of the RWTH University Hospital in Aachen, Germany
,
Norbert Pallua
Department of Plastic Surgery and Hand Surgery, Burn Unit of the RWTH University Hospital in Aachen, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
31 December 2019 (online)

ABSTRACT

Objective: Soluble CD163 (sCD163) has been previously shown to play a role in inflammatory and infectious diseases. This study, for the first time, investigates the characteristics and potential values of sCD163 in burn patients. A first look is taken on the changes of sCD163 levels in burn patients by comparing predefined subgroups at single time points. Materials and Methods: Serum samples of 18 patients with burn injuries were collected for biochemical analysis at the time of admission and in a chronological sequence of 12, 24, 48 and 120 h after the injury and were matched to clinical parameters. Statistical analysis was performed using the Mann-Whitney test, Wilcoxon signed rank and Pearson bivariate correlation. Results: Patients with sepsis showed a significant increase of sCD163 levels. sCD163 was correlated with leukocytes (P=0.035) over the time course of 120 h. Patients characterized by a burn size exceeding 25% of the total body surface area (TBSA) showed a significant increase of sCD163 between 12 and 48 h after burn injury (P=0.038). Conclusions: The first view on the characteristics of sCD163 in the serum of burn patients points out that sCD163 seems to be an early indicator for the susceptibility to sepsis. Furthermore, the changes in sCD163 serum levels within the first hours after burn trauma have great potential for early prediction of organ failure after burn injury.

 
  • REFERENCES

  • 1 Mosser DM. The many faces of macrophage activation. J Leukoc Biol 2003;73:209-12.
  • 2 Kristiansen M, Graversen JH, Jacobsen C, Sonne O, Hoffman HJ, Law SK, et al. Identification of the haemoglobin scavenger receptor. Nature 2001;409:198-201.
  • 3 Buechler C, Ritter M, Orso E, Langmann T, Klucken J, Schmitz G. Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuli. J Leukoc Biol 2000;67:97-103.
  • 4 Moestrup SK, Moller HJ. CD163. A regulated hemoglobin scavenger receptor with a role in the anti-inflammatory response. Ann Med 2004;36:347-54.
  • 5 Droste A, Sorg C, Hogger P. Shedding of CD163, a novel regulatory mechanism for a member of the scavenger receptor cysteine-rich family. Biochem Biophys Res Commun 1999;256:110-3.
  • 6 Sulahian TH, Hintz KA, Wardwell K, Guyre PM. Development of an ELISA to measure soluble CD163 in biological fluids. J Immunol Methods 2001;252:25-31.
  • 7 Moller HJ, Peterslund NA, Graversen JH, Moestrup SK. Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood 2002;99:378-80.
  • 8 Timmermann M, Hogger P. Oxidative stress and 8-iso-prostaglandin F(2alpha) induce ectodomain shedding of CD163 and release of tumor necrosis factor-alpha from human monocytes. Free Radic Biol Med 2005;39:98-107.
  • 9 Hintz KA, Rassias AJ, Wardwell K, Moss ML, Morganelli PM, Pioli PA, et al. Endotoxin induces rapid metalloproteinase-mediated shedding followed by up-regulation of the monocyte hemoglobin scavenger receptor CD163. J Leukoc Biol 2002;72:711-7.
  • 10 ACCP/SCCM Consensus Conference Committee. Definition for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. Crit Care Med 1992;20:864-74.
  • 11 Piatkowski A, Groger A, Pantel M, Bozkurt A, Fuchs PC, Pallua N. The extent of thermal injury affects fractions of mononuclear cells. Burns 2009;35:256-63.
  • 12 Tobiasen J, Hiebert JM, Edlich RF. The abbreviated burn severity index. Ann Emerg Med 1982;11:260-2.
  • 13 Gaini S, Pedersen SS, Koldkaer OG, Pedersen C, Moestrup SK, Moller HJ. New immunological serum markers in bacteraemia: Anti-inflammatory soluble CD163, but not proinflammatory high mobility group-box 1 protein, is related to prognosis. Clin Exp Immunol 2008;151:423-31.
  • 14 Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev 2006;19:403-34.
  • 15 Frings W, Dreier J, Sorg C. Only the soluble form of the scavenger receptor CD163 acts inhibitory on phorbol ester-activated T-lymphocytes, whereas membrane-bound protein has no effect. FEBS lett 2002;526:93-6.
  • 16 Hogger P, Sorg C. Soluble CD163 inhibits phorbol ester-induced lymphocyte proliferation. Biochem Biophys Res Commun 2001;288:841-3.
  • 17 Weaver LK, Pioli PA, Wardwell K, Vogel SN, Guyre PM. Up-regulation of human monocyte CD163 upon activation of cell-surface Toll-like receptors. J Leukoc Biol 2007;81:663-71.