Open Access
CC BY-NC-ND 4.0 · Int Arch Otorhinolaryngol 2020; 24(01): e47-e52
DOI: 10.1055/s-0039-1698782
Original Research
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin

1   Department of Phonoaudiology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
,
Aron Ferreira da Silveira
2   Department of Morphology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
,
Adriana de Andrade Batista Murashima
3   Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirao Preto, SP, Brazil
,
Maria Rossato
3   Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirao Preto, SP, Brazil
,
Miguel Angelo Hippolito
3   Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirao Preto, SP, Brazil
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Publikationsverlauf

04. Juli 2019

07. September 2019

Publikationsdatum:
09. Januar 2020 (online)

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Abstract

Introduction Cisplatin damages the auditory system and is related to the generation of free radicals. Glutathione peroxidase is an endogenous free radicals remover.

Objective To investigate the mechanisms involved in otoprotection by N-acetylcysteine through the expression of glutathione peroxidase in outer hair cells from rats treated with cisplatin.

Methods Male Wistar rats were intraperitoneally injected with cisplatin (8 mg/Kg) and/or received oral administration by gavage of N-acetylcysteine (300 mg/Kg) for 3 consecutive days. On the 4th day, the animals were euthanized and beheaded. The tympanic bullae were removed and prepared for scanning electron microscopy and immunofluorescence.

Results Among the groups exposed to ototoxic doses of cisplatin, there was an increase in glutathione peroxidase immunostaining in two groups, the one exposed to cisplatin alone, and the group exposed to both cisplatin and N-acetylcysteine.

Conclusion The expression of glutathione peroxidase in the outer hair cells of rats exposed to cisplatin showed the synthesis of this enzyme under cellular toxicity conditions.