CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2019; 54(05): 572-578
DOI: 10.1055/s-0039-1697016
Artigo Original | Original Article
Sociedade Brasileira de Ortopedia e Traumatologia. Published by Thieme Revnter Publicações Ltda Rio de Janeiro, Brazil

Effects of Mechanical Vibration in Neuromuscular Junctions and Fiber Type of the Soleus Muscle of Oophorectomized Wistar Rats[*]

Article in several languages: português | English
Ana Luiza Peretti
1  Universidade Estadual do Oeste do Paraná - Campus Cascavel, Cascavel, PR, Brasil
,
Camila Mayumi Martin Kakihata
1  Universidade Estadual do Oeste do Paraná - Campus Cascavel, Cascavel, PR, Brasil
,
Maria Luiza Serradourada Wutzke
1  Universidade Estadual do Oeste do Paraná - Campus Cascavel, Cascavel, PR, Brasil
,
Márcia Miranda Torrejais
1  Universidade Estadual do Oeste do Paraná - Campus Cascavel, Cascavel, PR, Brasil
,
Lucinéia de Fátima Chasko Ribeiro
1  Universidade Estadual do Oeste do Paraná - Campus Cascavel, Cascavel, PR, Brasil
,
1  Universidade Estadual do Oeste do Paraná - Campus Cascavel, Cascavel, PR, Brasil
› Author Affiliations
Further Information

Publication History

22 March 2018

10 July 2018

Publication Date:
23 September 2019 (online)

Abstract

Objective To evaluate the neuromuscular junctions (NMJs) and the type of muscle fibers of the soleus muscle of oophorectomized Wistar rats submitted to a mechanical vibration protocol.

Methods A total of 36 randomized rats were used in the pseudo-oophorectomy without and with treatment and oophorectomy without and with treatment groups. The treatment was performed with a vibratory platform, frequency of 60 Hz and duration of 10 minutes, 3 times a week, for 4 weeks. At the end of the intervention period, the animals were euthanized and the soleus muscles were collected and processed for analysis of the NMJs and fiber type. The data were analyzed for normality by the Shapiro-Wilk test and analysis of the 3-way variance using the post-hoc Tukey test, when necessary, and a significance level of 5% was adopted.

Results In the analysis of the NMJs, the oophorectomy group presented a smaller area than the pseudo-oophorectomy group, but the oophorectomy with treatment group was equal to the pseudo-oophorectomy with treatment group. For the larger diameter of the joints, the oophorectomy group was also different from the others; however, the oophorectomy and treatment animals were larger than those of the pseudo-oophorectomy and treatment group. There was no distinction of the types of fibers, with the muscle presenting fibers of the oxidative type.

Conclusion Hormonal deprivation reduced the area and diameter of the NMJs, with reversion of this process in the groups that underwent vibratory platform treatment for 4 weeks, and both surgery and treatment did not influence the type of soleus muscle fiber, composed of oxidative fibers.

* Work developed at Universidade Estadual do Oeste do Paraná (UNIOESTE) – Campus Cascavel, PR, Brazil