Thromb Haemost 2019; 119(12): 1994-2004
DOI: 10.1055/s-0039-1696972
Cellular Haemostasis and Platelets
Georg Thieme Verlag KG Stuttgart · New York

Premorbid Hemostasis in Women with a History of Pregnancy Loss

Alina D. Peshkova
1  Kazan Federal University, Kazan, Russia
Svetlana I. Safiullina
2  Kazan State Medical University, Kazan, Russia
3  Medical Center “Aibolit,” Kazan, Russia
Natalia G. Evtugina
1  Kazan Federal University, Kazan, Russia
Yelena S. Baras
4  Dar a Luz Birth and Health Center, Albuquerque, New Mexico, United States
Fazoil I. Ataullakhanov
5  Center for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of Sciences, Moscow, Russia
John W. Weisel
6  School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States
Rustem I. Litvinov
1  Kazan Federal University, Kazan, Russia
6  School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States
› Author Affiliations
Funding This work was supported by NIH grants HL135254, U01HL116330, NSF grant DMR1505662, the Program for Competitive Growth at Kazan Federal University, grants 19–015–00075, 18–415–160004, and 19–51–15004 from the Russian Foundation for Basic Research, a grant of the Ministry of Science and Higher Education of the Russian Federation (project АААА-А18–118012390250–0), and a grant of the Presidium of the Russian Academy of Sciences (the Program for fundamental research “Fundamentals of the technology of physiological adaptations”).
Further Information

Publication History

23 May 2019

19 July 2019

Publication Date:
06 October 2019 (online)


Background Congenital and acquired hemostatic disorders are among the pathogenic factors of pregnancy loss. Studying mechanistic relations between impaired hemostasis and fetal losses is important for the prognosis and prophylaxis of obstetric complications.

Objective This article aims to establish latent hemostatic disorders in nonpregnant women as an important premorbid risk factor of pregnancy loss.

Methods and Results Hemostasis was characterized using two relatively new in vitro assays, namely thrombodynamics (spatial clot growth) and kinetics of blood clot contraction, which together reflect the hemostatic or thrombotic potential. In addition, platelet functionality was assessed using flow cytometry. Our study included 50 women with a history of pregnancy loss and 30 parous women without previous obstetric complications. In patients with pregnancy loss, hypercoagulability was observed along with significant impairment of blood clot contraction associated with chronic platelet activation and dysfunction. Both hypercoagulability and defective clot contraction were significantly more pronounced in patients with a history of three or more miscarriages compared with patients with a history of one or two miscarriages. In addition, a significant inhibition of clot contraction was found in patients with miscarriage occurring after 10 weeks of gestation compared with those who lost a fetus earlier in pregnancy.

Conclusion These results indicate that chronic hypercoagulability and impaired clot contraction constitute a premorbid status in patients with pregnancy loss. The data confirm a significant pathogenic role of hemostatic disorders in pregnancy loss and suggest the predictive value of thrombodynamics and blood clot contraction assays in evaluating the risk of pregnancy loss.

Authors' Contributions

R.I.L., S.I.S., F.I.A., and J.W.W designed the research; A.D.P. and N.G.E. performed experiments; A.D.P., S.I.S., Y.S.B., F.I.A., J.W.W., and R.I.L. analyzed the data; A.D.P., J.W.W., and R.I.L. wrote the paper.

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