Download PDF
CC BY-NC-ND 4.0 · International Journal of Epilepsy 2018; 05(02): S14
DOI: 10.1055/s-0039-1694893
Abstracts of 20th Joint Annual Conference of Indian Epilepsy Society and Indian Epilepsy Association (ECON 2019)
Indian Epilepsy Society

Preclinical Screening of Antiepileptic Properties of Diltiazem in Chemically and Electrically Induced Battery of Seizures Tests in Laboratory Experimental Animal Models

Saniya K.‡
1   Department of Anatomy, All India Institute of Medical Sciences, Delhi, India
,
Patil B. G.‡
1   Department of Anatomy, All India Institute of Medical Sciences, Delhi, India
,
Madhavrao C.‡
2   Department of Pharmacology, All India Institute of Medical Sciences, Delhi, India
,
Prakash K. G.‡
1   Department of Anatomy, All India Institute of Medical Sciences, Delhi, India
,
Mythili Bai K.‡
3   Department of Physiology, All India Institute of Medical Sciences, Delhi, India
› Author Affiliations
Subject Editor:
Further Information

Publication History

Publication Date:
31 July 2019 (online)

Also available at
  Permissions and Reprints

Objective: To screen the antiepileptic properties of diltiazem in chemically and electrically induced battery of seizures tests in laboratory experimental animal models.

Methods: In this study, Wistar albino rats were used as experimental animal models. The proposal was approved by the official bodies including Institutional Research Committee (IRC) and Institutional Animal Ethics Committee (IAEC). Experimental techniques were performed adhering to the CPCSEA guidelines. This study had a total of six groups, each group had six animals and the groups were group I: control for PTZ, group II: standard for PTZ (sodium valproate), group III: diltiazem for PTZ, group IV: control for MES, group V: standard for MES (diphenylhydantoin), and group VI: diltiazem for MES. Various statistical tests including one-way ANOVA followed by Bonferroni's post hoc test and Kruskal–Wallis test followed by Dunn's post hoc test were applied, wherever applicable to find the statistically significance levels.

Results: It was seen that, the experimental test drug (diltiazem) was able to increase the onset of seizures when compared with the control group (group I) with p < 0.001. Similarly, the standard drug in group II also had similar Results. Other parameters like duration of seizures, number of seizures in 1 hour and score of seizures were significantly reduced in both the experimental test and standard drugs (groups II and III) when compared with the control group (p < 0.05). The obtained above Results in group II were comparable to group III. In electrically induced MES model, as expected in group V, none of the animals exhibited Tonic Hind Limb Extension (THLE). In diltiazem experimental test drug group, more than 80% of animals were protected against the THLE. Again in this model, there was a significant reduction in scores of seizures in both the groups (V and VI) when compared with their parent control group (p < 0.01).

Conclusion: Diltiazem exhibited significant antiepileptic properties in chemically and electrically induced battery of seizures tests in laboratory experimental animal models.