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CC BY-NC-ND 4.0 · Eur J Dent 2019; 13(02): 238-242
DOI: 10.1055/s-0039-1694697
Original Article
Dental Investigation Society

Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells

Sri Wigati Mardi Mulyani
1   Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia
,
Eha Renwi Astuti
1   Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia
,
Otty Ratna Wahyuni
1   Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia
,
Diah Savitri Ernawati
2   Department of Oral Medicine, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia
,
Nastiti Faradilla Ramadhani
1   Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia
› Author Affiliations
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Publication History

Publication Date:
11 September 2019 (online)

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Abstract

Objectives The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O2 concentration.

Materials and Methods Stem cell culture was performed under normoxic (O2: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N2, 5% CO2, and 1% O2. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation.

Statistical Analysis Statistical analysis was done using normality test, followed by MANOVA test (p < 0.05).

Results There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (p < 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia.

Conclusion BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.

Keywords

bone marrow mesenchymal stem cells - hypoxic precondition - salivary gland defect - SDF1-CXCR4 - Bcl-2 - α-amylase
 

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