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CC BY-NC-ND 4.0 · Eur J Dent 2019; 13(02): 199-205
DOI: 10.1055/s-0039-1694307
Original Article
Dental Investigation Society

Effect of Dentin Pretreatment with Arginine on Microshear Bond Strength of Etch-and-Rinse or Self-Etch Adhesive Systems

Gabriela Oliveira Borgo
1   Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
,
Waldemir Francisco Vieira-Junior
1   Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
2   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Dental Research Center, Campinas, São Paulo, Brazil
3   School of Health Sciences, Centro Universitário das Faculdades Metropolitanas Unidas, São Paulo, Brazil
,
Jéssica Dias Theobaldo
1   Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
3   School of Health Sciences, Centro Universitário das Faculdades Metropolitanas Unidas, São Paulo, Brazil
,
Flávio Henrique Baggio Aguiar
1   Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
,
Débora Alves Nunes Leite Lima
1   Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
› Institutsangaben
Funding This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
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Publikationsdatum:
02. September 2019 (online)

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Abstract

Objective The main purpose of this study was to evaluate the effect of dentin pretreatment with 8% arginine on the immediate and 6-month bond strength (BS) of adhesive systems and surface morphology of dentin.

Materials and Methods Dentin bovine specimens (5 × 5 mm) were allocated into following pretreatments: without pretreatment (conventional technique, C) or exposure to arginine solution (A) for 5 minutes prior to tooth restoration. The adhesive procedure was performed using (n = 12): 3-step etch-and-rinse adhesive (Scotchbond Multipurpose—SBMP [C+SBMP and A+SBMP]); 2-step etch-and-rinse adhesive (Single Bond 2 - SB [C+SB and A+SB]); or 2-step self-etch adhesive (Clearfil SE Bond - CSEB [C+CSEB and A+CSEB]). After 24 hours, the composite resin restorations were evaluated immediately and after 6 months of water storage using a microshear test and fracture pattern. The dentin surface exposed to A was assessed by scanning electron microscopy (SEM). The immediate BS data were submitted to a two-way analysis of variance and Tukey's test, and the long-term BS results were analyzed using Kruskal–Wallis and Dunn tests (α = 0.05).

Results There was a significant decrease in immediate and 6-month BS for the A+SB and A+CSEB groups, which differed statistically from the C group. The fracture pattern was predominantly adhesive for SB and CSEB adhesive for C and A. The SEM images presented a different conditioning pattern of the dentin exposed to A.

Conclusion The dentin pretreatment with arginine interfered negatively in the immediate and long-term BS of the simplified adhesive system. However, the SBMP adhesive was not affected by arginine pretreatment presenting the most satisfactory results.

Keywords

arginine - dentin - adhesive systems - adhesion - dentin-bonding agents