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CC BY-NC-ND 4.0 · Eur J Dent 2019; 13(03): 432-436
DOI: 10.1055/s-0039-1693923
Original Article
Dental Investigation Society

Medicinal Signaling Cells Metabolite Oral Based as a Potential Biocompatible Biomaterial Accelerating Oral Ulcer Healing (In Vitro Study)

Alexander Patera Nugraha
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
2   Orthodontics Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
3   Doctoral Student of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Purwati,
Helen Susilowati
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
,
Eryk Hendrianto
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
,
Deya Karsari
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
,
Nora Ertanti
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
,
Aristika Dinaryanti
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
,
Igo Syaiful Ihsan
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
,
Ida Bagus Narmada
2   Orthodontics Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Diah Savitri Ernawati
4   Oral Medicine Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fedik Abdul Rantam
1   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Indonesia
5   Virology and Immunology Laboratory, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations
Further Information

Publication History

Publication Date:
03 December 2019 (online)

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Abstract

Objective Medicinal signaling cells metabolite (MSCM) is often considered medical waste even though it contains abundant growth factors, and advantageous micro- and macromolecules that can accelerate healing in oral ulcer.

The purpose of this experimental laboratory study was to analyze the biocompatibility and potential of MSCM, (oral based) to accelerate healing in oral ulcer (in vitro).

Materials and Methods MSCM (oral based) was obtained by mixing 10 mL of MSCM and 2% of carboxymethyl cellulose sodium. 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (or MTT assay) was obtained using human gingival somatic cell culture to examine cell viability treated with MSCM (oral based). Fourier transform infrared spectroscopy was performed to know the functional structure and composition of MSCM (oral based). To know the elemental composition of MSCM (oral based), energy-dispersive X-ray analysis was performed. Scratch test was performed to know the ability of MSCM (oral based) to increase human somatic cell proliferation.

Results MSCM (oral based) has good cell viability. MSCM (oral based) administration accelerated the proliferation of human somatic cell culture after 12-hours in vitro. MSCM (oral based) has carboxylic acids and derivatives chemical bond. MSCM (oral based) mostly contained carbon and potassium but did not contain heavy metal substances.

Conclusions MSCM (oral based) has a biocompatible and potential ability to accelerate healing in oral ulcer in vitro. It would be useful in daily clinical practice in treating traumatic oral ulcer.

Keywords

medicinal signaling cells - wound healing - biomaterial - metabolite - biocompatibility
 

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