Am J Perinatol 2020; 37(11): 1160-1172
DOI: 10.1055/s-0039-1692715
Original Article

Exploration of the Vaginal and Gut Microbiome in African American Women by Body Mass Index, Class of Obesity, and Gestational Weight Gain: A Pilot Study

1  Louise Herrington School of Nursing, Midwifery Specialty, Baylor University, Dallas, Texas
,
Kristen Leigh Greathouse
2  Department of Nutrition Science, Baylor University, Waco, Texas
,
Marie Hastings-Tolsma
1  Louise Herrington School of Nursing, Midwifery Specialty, Baylor University, Dallas, Texas
,
Robert Noah Padgett
3  Department of Educational Psychology, Baylor University, Waco, Texas
,
Kimberly Sakovich
4  Women's Health Care, MacArthur Obstetrics and Gynecology, Irving, Texas
,
Ankan Choudhury
5  Department of Biology, Baylor University, Waco, Texas
,
Aadil Sheikh
5  Department of Biology, Baylor University, Waco, Texas
,
Nadim J. Ajami
6  Department of Molecular Virology and Microbiology, Baylor College of Medicine, Texas Medical Center, Houston, Texas
,
Joseph F. Petrosino
7  The Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Texas Medical Center, Houston, Texas
› Institutsangaben
Funding This study was funded by the Collaborative Faculty Research Innovation Program (CFRIP) grant from Baylor University, Baylor College of Medicine, and Baylor Research Institution-Project number 30300146.

Abstract

Objective This study determines the differences in the distal gut and vaginal microbiome in African American (AA) women by prepregnancy body mass index and gestational weight gain (GWG) comparing women with and without obesity and by obesity class.

Study Design We prospectively sampled the vaginal and distal gut microbiome in pregnant AA women at two time points during pregnancy. Samples were analyzed using high-throughput sequencing of the V4 region of the 16S ribosomal ribonucleic acid gene.

Results Distinct differences in vaginal and distal gut α-diversity were observed at time point 1 between women with and without obesity by total GWG. Significant differences in distal gut β-diversity were also found at time point 1 in obese women by GWG. Within the Bacteroides genus, a significant association was observed by total GWG among obese women which was absent in nonobese women. Women with class III obesity who experienced low GWG had the lowest abundance of distal gut Bacteroides and appreciably higher relative abundance of a consortia of vaginal taxa including Atopobium, Gardnerella, Prevotella, and Sneathia.

Conclusion These results contribute new evidence showing that GWG in combination with obesity and obesity class is associated with an altered distal gut and vaginal composition early in pregnancy among AA women.

Declaration of Data Set

The data set supporting the conclusions of this article included within the article and its' additional files is available in the BioProject database/ repository, BioProject ID: PRJNA422447, link: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ncbi.nlm.nih.gov_bioproject_422447&d=DwICaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=YTUxyBdU_6BctUsXVw5ZzwpbWswno7FbIZTkWuuKt1s&m=uyeGLBv-Cc4pPpaIxdDJJD_q_5mZCJm0YtPkeYRPCVg&s=urOK5llZoIIt2FtH9WZjPyIJdc6PassC4uGkIJumozM&e=.


Authors' Contributions

M.A.F., K.L.G., M.H.T., K.S., N.A., and J.P. have taken part in the study design. M.A.F., K.L.G., M.H.T., A.C., A.S., N.A., R.N.P., and J.P. have contributed to the interpretation of the data, manuscript preparation, and/or review and approval of the final version. M.A.F. conceived the study design. M.A.F., M.H.T., and K.S. collected samples.


Supplementary Material



Publikationsverlauf

Eingereicht: 28. Januar 2019

Angenommen: 14. Mai 2019

Publikationsdatum:
26. Juni 2019 (online)

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