Hypermethylation of the MTHFRgene is association with cardio metabolic complications of T2DM subjects

Question

The hypermethilation of the MTHFgene is a great concern regarding to T2DM complications.Additionaly, quite a few studies tried to correlate of this enzyme gene polymorphisms with diabetic risk factoprs, but very little were known regarding the relationship of this gene mutation with cardiometabolic complication of diabetes. The objective to conduct this study was to evaluate the association in between methylation profiling of the MTHFR gene promoter and its correlation with cardiometabolic markers such as inflammatory, biochemical and oxidative stress among the subjects with type 2 diabetes (T2DM).

Methods:

The T2DM patients blood leucocyte DNA, MTHFR methylation profile was analysed by using the PCR technique for methylation (MSP).The anthropometric parameters, BMI, HC, WC, bloodpresuure were measured bu the standard techniques. The cardiometabolic markers such as HbA1c, total cholesterol, TG, LDL, HDL, C-reactive protein, total antioxidant and malonaldehyde were analyzed. The Mann-Whitney statistical approach was used to evaluate the correlation, p < 0.05 was considered as significant.

Results:

The hypermethylated profile of the MTHFRgene was found in T2DM individuals with with higher total cholesterol and low HDL levels p 0.001 and 0.00267, respectively. The correlation was also found with low total antioxidant capacity, higher CRP and the indivisulas with higher BMI and WC individually.

Conclusions:

The hypermethylation act as the promoter of the MTHFRgene in association with the occurrence of cardiometabolic complications.This can be used to detection of the early cardiometabolic complication in patients with T2DM