Diabetologie und Stoffwechsel 2019; 14(S 01): S42
DOI: 10.1055/s-0039-1688227
Poster
Entzündung und mehr
Georg Thieme Verlag KG Stuttgart · New York

Role of tryptophan metabolites in metabolic inflammation and type 2 diabetes

J Brandes
1   Uniklinik Schleswig-Holstein, Department of Medicine 1, Kiel, Germany
,
D Fangmann
1   Uniklinik Schleswig-Holstein, Department of Medicine 1, Kiel, Germany
,
F Brix
2   Institute of Human Nutrition and Food Science, University of Kiel, Germany, Division of food technology, Kiel, Germany
,
T Demetrowitsch
2   Institute of Human Nutrition and Food Science, University of Kiel, Germany, Division of food technology, Kiel, Germany
,
J Jensen-Kroll
2   Institute of Human Nutrition and Food Science, University of Kiel, Germany, Division of food technology, Kiel, Germany
,
K Hartmann
1   Uniklinik Schleswig-Holstein, Department of Medicine 1, Kiel, Germany
,
K Türk
1   Uniklinik Schleswig-Holstein, Department of Medicine 1, Kiel, Germany
,
S Schreiber
1   Uniklinik Schleswig-Holstein, Department of Medicine 1, Kiel, Germany
,
K Schwarz
2   Institute of Human Nutrition and Food Science, University of Kiel, Germany, Division of food technology, Kiel, Germany
,
M Laudes
1   Uniklinik Schleswig-Holstein, Department of Medicine 1, Kiel, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
07 May 2019 (online)

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Introduction:

Aromatic amino acids, especially tryptophan (Trp), are reported to be important biomarkers to identify human subjects at high risk for type 2 diabetes (T2D). Moreover, some metabolites of the Niacin-pathway, like nicotinuric acid (NUA), exhibit pro-inflammatory capacity by promoting polarization of M2 in M1 macrophages. The aim of the study was to use non-targeted metabolomics analysis to further characterize the Trp/niacin- metabolism in metabolic inflammation.

Patients and methods:

A sub-cohort of 600 male and female subjects (age 23 – 89) from the Food-Chain-Plus cohort (FoCus) was selected. The subjects were stratified in 200 healthy, 200 prediabetes (defined by impaired fasting glucose = IFG) and 200 patients of T2D. Trp – and niacin metabolites were measured by FT-ICR-MS. The evaluation was conducted with MetaboScape (Bruker, Germany).

Results:

Trp- metabolites kynurenine, anthranilic acid and Trp itself were found in sub-cohort samples. In addition, 18 further compounds of niacin metabolism, like NUA, were identified. Investigation showed especially for Trp and NUA a noticeable difference in intensity. While groups of IFG and T2D showed higher intensities of Trp and NUA, the controls had a considerably lower intensity of these metabolites.

Conclusion:

This investigation supports previous studies that Trp has the ability to predict onset of T2D. Remarkable intensity differences of NUA between healthy subjects and persons with IFG or T2D suggest that increasing intensity of this metabolite provides a shift from M2 to M1-macrophages and therefore reinforces low-grade inflammation.