Detection of cannabinoide receptors 1 and 2 in human nasale mucosa – a pilot study

Introduction:

The Endocannbinoidsystem (ECS) has been recently identified in inflammatory reactions. CB1-receptors are mainly present in central nervous system. CB2-Receptors has been identified on inflammatory peripheral immune cells (e.g. T cells, B cells and mast cells).

Methods:

15 samples of patient undergoing endoscopic sinus surgery (CRSwNP and CRSsNP) were evaluated. Western blot was applied to detect CB1 and CB2 receptors on level of protein. To overrun the results, the real-time PCR was performed on subgroups by endotyping of patients with multiplex Elisa.

Results:

15 samples have been examined in human nasal mucosa. Two groups were set – group 1: chronic rhinosinusitis with nasal polyps n = 11, group 2: chronic rhinosinusitis without nasal polyps n = 4. Both receptors were detected in control tissue. CB1 receptors were more present in the group of chronic rhinosinusitis without polyps and CB2 receptors in the group of chronic rhinosinusitis with polyps.

Conclusion:

We were able to demonstrate CB1 and CB2 receptors in the human nasal mucosa in an in vitro experimental model by application of a quantitative analysis. With the detection of cannabinoid receptors, another interesting aspect opens up to explore the pathophysiology of CRS in the coming years.

Publication History

Publication Date:
23 April 2019 (online)