CC BY-NC-ND 4.0 · Laryngorhinootologie 2019; 98(S 02): S263
DOI: 10.1055/s-0039-1686016
Poster
Oncology

Physical characterization of HNSCC cells

J Kristin
1   Hals-Nasen-Ohrenklinik, Uniklinik Düsseldorf, Düsseldorf
,
M Strugacevac
2   Institut für angewandte Physik, Heinrich-Heine-Universität Düsseldorf, Düsseldorf
,
HC Tsai
3   Institut für angewandte Physik, Heinrich-Heine Universität, Düsseldorf, Düsseldorf
,
N Ullrich
3   Institut für angewandte Physik, Heinrich-Heine Universität, Düsseldorf, Düsseldorf
,
C Wiek
1   Hals-Nasen-Ohrenklinik, Uniklinik Düsseldorf, Düsseldorf
,
J Schipper
1   Hals-Nasen-Ohrenklinik, Uniklinik Düsseldorf, Düsseldorf
,
M Getzlaff
3   Institut für angewandte Physik, Heinrich-Heine Universität, Düsseldorf, Düsseldorf
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Introduction:

Physical properties of cells, e.g. elasticity, are largely determined by the cytoskeleton. The aim of this study was to determine physical differences between healthy and carcinoma cells of the head and neck area. The results will serve as a basis for the detection of specific sound frequencies that selectively ablate malignant cells and protect healthy cells.

Material and method:

The investigated cell lines are a squamous cell carcinoma cell line (UD-SCC-1, oropharynx) and a healthy oral keratinocyte cell line (HOK, ScienCell). The volume of the cellular cytoskeleton in comparison to the total cell volume was determined using a self-developed Matlab script. The elasticity of healthy and carcinoma cells was measured by atomic force microscopy.

Results:

The Matlab script showed a proportion of actin filaments in the carcinoma cell line of 48%± 10% compared to 49%± 12% in the healthy keratinocyte cell line. The proportion of microtubules was 55%± 12% in the carcinoma cells and 44%± 10% in the keratinocyte cell line. The carcinoma cell line has an elasticity of 2.58 kPa, the keratinocyte cell line an average elasticity of 4.59 kPA.

Conclusions:

While healthy keratinocytes differ from the examined carcinoma cell line in their elasticity, no difference of all examined cytoskeletal components could be shown.



Publication History

Publication Date:
23 April 2019 (online)

© 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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