J Pediatr Infect Dis 2019; 14(04): 194-200
DOI: 10.1055/s-0039-1685502
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Presence of Extended-Spectrum β-lactamase, CTX-M-65 in Salmonella enterica serovar Infantis Isolated from Children with Diarrhea in Lima, Peru

Ana Granda
1   Department of Pediatrics, Instituto de Medicina Tropical “Alexander von Humboldt,” Universidad Peruana Cayetano Heredia, Lima, Perú
Maribel Riveros
1   Department of Pediatrics, Instituto de Medicina Tropical “Alexander von Humboldt,” Universidad Peruana Cayetano Heredia, Lima, Perú
Sandra Martínez-Puchol
2   ISGlobal, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain
Karen Ocampo
1   Department of Pediatrics, Instituto de Medicina Tropical “Alexander von Humboldt,” Universidad Peruana Cayetano Heredia, Lima, Perú
Laura Laureano-Adame
3   Food Research Center, Nutreco, Toledo, Spain
Alfredo Corujo
3   Food Research Center, Nutreco, Toledo, Spain
Isabel Reyes
4   Unidad de investigación pediátrica, Hospital de Emergencias Pediátricas, Lima, Perú
2   ISGlobal, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain
Theresa J. Ochoa
1   Department of Pediatrics, Instituto de Medicina Tropical “Alexander von Humboldt,” Universidad Peruana Cayetano Heredia, Lima, Perú
5   Center for Infectious Diseases, University of Texas Health Science Center at Houston, Houston, TX, United States
› Author Affiliations
Funding J. R. was supported by the I3 program of the Ministerio de Economia y Competitividad, Spain (grant number: CES11/012). ISGlobal is a member of the CERCA Programme, Generalitat de Catalunya.
Further Information

Publication History

17 July 2018

07 March 2019

Publication Date:
20 April 2019 (online)


Gastroenteritis in children is a serious condition in many parts of the world. Salmonella enterica is one of the causes of the disease. In this study, 280 fecal samples from children with diarrhea in four hospitals in Lima, Peru, were collected between September 2012 and March 2013. Salmonella was detected in 26 of the samples. Serotyping demonstrated that 25 of the isolates were S. enterica Infantis, and one isolate was S. enterica Typhimurium. Repetitive extragenic palindromic–polymerase chain reaction analysis suggests that all S. Infantis belong to the same clone. All but one of the S. Infantis isolates exhibited an extended-spectrum β-lactamase phenotype as they harbored bla CTX-M 65. Two strains also carried bla TEM-1. Nine of the isolates were resistant to azithromycin and two to ciprofloxacin. This study demonstrates that a multidrug-resistant S. Infantis clone carrying bla CTX-M 65 was circulating among children in Lima, Peru. The development of molecular epidemiology studies in Salmonella-causing diarrhea or other pathologies in Lima and in other areas will be useful to determine the permanence, geographical spread, and clinical implications of this clone.

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