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Thromb Haemost 1979; 42(01): 54
DOI: 10.1055/s-0039-1684392
Activation of Prothrombin and Factor X
Poster Board
Schattauer GmbH

A Chromogenic Factor X Assay with New Standardized Reagents

P. Friberger
1   AB KABI, Peptide Research, Mölndal, Sweden.
,
C. Lenne
1   AB KABI, Peptide Research, Mölndal, Sweden.
› Author Affiliations
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Publication History

Publication Date:
18 April 2019 (online)

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A recently published method for Factor X (FX) assay (1) utilizing Russel’s Viper Venom (RW) and a chromogenic substrate has been further investigated by testing a large number of parameters, This method has been considered as a suitable method for monitoring coumarol treatment (Bergström et al).

The conditions for the activation of FX by purified preparations of the RVV have been studied as well as the conditions for FXa determination with a new chromogenic substrate Bz-Ile-Glu(γ-piperidyl)-Gly-Arg-pNA (S-2337). Both purified factors and normal plasma have been used. The effect of plasma inhibitors as well as the selectivity of the method bas been studied.

The reproducibility and stability of the different reagents and standards have been studied and found to be good.

The amount of FXa activity obtained from normal human plasma has been titrated with FXa inhibitors of known purity.

1) Aurell L. et al, Thromb. Res., 11, 595 (1977)

2) Bergström et al, Thromb. Res., 12, 531 (1978)