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DOI: 10.1055/s-0039-1684391
Interaction of Prothrombin with Phospholipids Monolayers in Presence of Ca**.
Publication History
Publication Date:
18 April 2019 (online)

The activation rate of Prothrombin and its conversion into thrombin through a proteolytic degradation is enhanced by phospholipids in the presence of Ca++. In this work, we could elucidate stoechiomet rio and structural aspects of the interaction between prothrombin. phospholipids and Ca++. We studied the adsorption equilibria of Ca++ and of prothrombin un phospholipids monolayers by a new approach which comoines two complementary methods of surface measurement.
Electrochemical investigations on lipid monolayer interacting with prothrombin showed penetration of prothrombin in presence or not of Ca++ (by increasing the lipid layer capacitance). with a preferential orientation. The adsorption of Ca++ and prothrombin to the lipid layer was measured by counting the surface radioactivity of 45Ca and 3H-prothrombin. Synergetic attachment of Ca++ and prothrombin to the phospholipid water interface was observed and the number of Ca++ per attached prothromoin was determinen. The two methods give comparative results for the proposed ionic-hydrophobic interaction.