Phenomenological Characterization of Low-Potency Homeopathic Preparations by Means of Pattern Formation in Evaporating DropletsFunding This investigation was funded by institutional sources only.
05 July 2018
05 October 2018
09 January 2019 (eFirst)
Background Evaporation-induced pattern formation in droplets has been applied to test effects of high potencies. Here we propose for the first time the droplet evaporation method (DEM) as a tool to characterize low potencies on a qualitative and quantitative basis.
Materials and Methods The present investigation consisted of: (1) screening of 18 different substances of mineral, vegetal, and animal origin in the 1x to 6x potency range; choice of four substances with characteristic pattern-forming properties; (2) replication experiments aiming at the differentiation of four homeopathic preparations at the same potency levels (2x–6x); and (3) control experiments performed on three preparations. The DEM experimental protocol consisted of the evaporation of droplets of the analyzed potency per se, placed on microscope slides and in controlled conditions. The resulting patterns were photographed and subjected to computerized image analysis.
Results The screening experiments yielded a wide variety of patterns. Homeopathic preparations of mineral origin showed the largest variety of forms, whereas potencies of vegetal origin mostly created dendritic patterns, probably due to diffusion-limited aggregation. The here-analyzed image analysis variables (gray-level distribution, entropy, and inverse difference moment) allowed a highly significant differentiation of patterns prepared from four substances (Echinacea, Baptisia, Luffa, and Spongia) at the same potency levels in the range 2x to 4x, whereas patterns obtained from potencies 5x and 6x could no longer be differentiated and resembled the pattern of pure solute (purified water). The control experiments showed reasonable experimental model stability.
Conclusions DEM seems to be a promising tool for qualitative phenomenological characterization of homeopathic preparations in low potency. We propose the application of the current experimental model for investigating further research topics in this field, such as the comparison of potencies versus simple dilutions or the contribution of component remedies to the patterns formed by homeopathic combination medicines.
Keywordsdroplet evaporation method - crystallization patterns - homeopathy - mineral extracts - vegetal extracts - low potencies
• Droplet evaporation patterns from potencies of mineral origin show a great variety of forms.
• Patterns from potencies of vegetal origin show rather dendritic structures typical for diffusion-limited aggregation.
• Computerized pattern evaluation allowed us to differentiate between potencies deriving from different substances until 4x.
• Patterns from 5x and 6x potencies resembled the pattern of control water.
• The droplet evaporation method might serve to analyze the matter still present in low potencies and how it changes during consecutive potentization steps.
- 1 Hahnemann S. Organon of Medicine. 6th ed. London: Headland; 1849
- 2 Kokornaczyk MO, Scherr C, Bodrova NB, Baumgartner S. Phase-transition induced pattern formation applied to basic research in homeopathy: a systematic review. Homeopathy 2018; 107: 181-188
- 3 Kokornaczyk MO, Trebbi G, Dinelli G. , et al. Droplet evaporation method as a new potential approach for highlighting the effectiveness of ultra high dilutions. Complement Ther Med 2014; 22: 333-340
- 4 Kokornaczyk MO, Baumgartner S, Betti L. Preliminary study on force-like effects between As45x, water, and wheat seeds performed by means of the droplet evaporation method. Int J High Dilution Res 2015; 14: 17-19
- 5 Kokornaczyk MO, Baumgartner S, Betti L. Polycrystalline structures formed in evaporating droplets as a parameter to test the action of Zincum metallicum 30c in a wheat seed model. Homeopathy 2016; 105: 173-179
- 6 Betti L, Trebbi G, Kokornaczyk MO. , et al. Number of succussion strokes affects effectiveness of ultra-high-diluted arsenic on in vitro wheat germination and polycrystalline structures obtained by droplet evaporation method. Homeopathy 2017; 106: 47-54
- 7 Elia V, Ausanio G, Gentile F, Germano R, Napoli E, Niccoli M. Experimental evidence of stable water nanostructures in extremely dilute solutions, at standard pressure and temperature. Homeopathy 2014; 103: 44-50
- 8 Lo SY, Geng X, Gann D. Evidence for the existence of stable-water-clusters at room temperature and normal pressure. Phys Lett A 2009; 373: 3872-3876
- 9 European Pharmacopoeia, Ninth Ed, Supplement 9.4. EDQM, editor. Strasbourg, France: Council of Europe; 2017
- 10 Medpharm Scientific Publishers. German Homoeopathic Pharmacopoeia (GHP) – including 14th Supplement. Stuttgart, Germany: Medpharm Scientific Publishers; 2017
- 11 Collins TJ. ImageJ for microscopy. Biotechniques 2007; 43: 25-30
- 12 Cabrera JE. Texture Analyzer. 2006. Available at: https://imagej.nih.gov/ij/plugins/texture.html . Accessed April 11, 2018
- 13 Carmer SG, Swanson MR. An evaluation of ten pairwise multiple comparison procedures by Monte Carlo Methods. J Am Stat Assoc 1973; 68: 66-74
- 14 Waychunas GA. Structure, aggregation and characterization of nanoparticles. Rev Mineral Geochem 2001; 44: 105-166
- 15 van Enckevort WJP, Los JH. On the creeping of saturated salt solutions. Cryst Growth Des 2013; 13: 1838-1848
- 16 Lee WP, Routh AF. Why do drying films crack?. Langmuir 2004; 20: 9885-9888
- 17 Sosso GC, Chen J, Cox SJ. , et al. Crystal nucleation in liquids: open questions and future challenges in molecular dynamics simulations. Chem Rev 2016; 116: 7078-7116
- 18 Wu J, Li Z. Density-functional theory for complex fluids. Annu Rev Phys Chem 2007; 58: 85-112
- 19 Kokornaczyk MO, Dinelli G, Marotti I, Benedettelli S, Nani D, Betti L. Self-organized crystallization patterns from evaporating droplets of common wheat grain leakages as a potential tool for quality analysis. Scientific World Journal 2011; 11: 1712-1725
- 20 Kokornaczyk MO, Dinelli G, Betti L. Approximate bilateral symmetry in evaporation-induced polycrystalline structures from droplets of wheat grain leakages and fluctuating asymmetry as quality indicator. Naturwissenschaften 2013; 100: 111-115