Sarcolemmal Alterations in Unloaded Rat Heart after Heterotopic Transplantation

Naoki Makino; Paul Ganguly; Vijayan Elimban; Naranjan S. Dhalla

doi:10.1055/s-0038-1673646

International Journal of Angiology, Table of Contents

Int J Angiol 2018; 27(04): 196-201
DOI: 10.1055/s-0038-1673646

Original Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Sarcolemmal Alterations in Unloaded Rat Heart after Heterotopic Transplantation

Naoki Makino

¹Department of Molecular and Clinical Gerontology, Medical Institute of the Bioregulation, Kyushu University, Oita, Japan

,

Paul Ganguly

²Department of Anatomy, College of Medicine, Alfaisal University, Riyadh, Kingdom of Saudi Arabia

,

Vijayan Elimban

³Department of Physiology and Pathophysiology, Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, University of Manitoba, Winnipeg, Canada

,

Naranjan S. Dhalla

³Department of Physiology and Pathophysiology, Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, University of Manitoba, Winnipeg, Canada

› Author Affiliations

Abstract

Following heterotopic transplantation, the rat heart undergoes atrophy and exhibits delayed cardiac relaxation without any changes in contraction and systolic Ca²⁺ transients. Furthermore, the sarcoplasmic reticular Ca²⁺ uptake and release activities were reduced and Ca²⁺ influx through L-type Ca²⁺ channels was increased in the atrophied heart. Since Ca²⁺ movements at sarcolemma are intimately involved in the regulation of intracellular Ca²⁺ concentration, the present study was undertaken to test if sarcolemma plays any role to maintain cardiac function in the atrophied heart.The characteristics of sarcolemmal Ca²⁺ pump and Na⁺–Ca²⁺ exchange activities were examined in 8 weeks heterotopically isotransplanted rat hearts which did not support hemodynamic load and underwent atrophy. Sarcolemmal ATP (adenosine triphosphate)-dependent Ca²⁺ uptake and Ca²⁺-stimulated ATPase (adenosine triphosphatase) activities were increased without any changes in Na⁺–K⁺ ATPase activities in the transplanted hearts. Although no alterations in the Na⁺-dependent Ca²⁺ uptake were evident, Na⁺-induced Ca²⁺ release was increased in the transplanted heart sarcolemmal vesicles. The increase in Na⁺-induced Ca²⁺ release was observed at different times of incubation as well as at 5, 20, and 40 mM Na⁺. The sarcolemma from transplanted hearts also showed higher contents of phosphatidic acid, sphingomyelin, and cholesterol.These results indicate that increases in the sarcolemmal, Ca²⁺ transport activities in unloaded heart may provide an insight into adaptive mechanism to maintain normal contractile behavior of the atrophic heart.

Keywords

cardiac contractility - sarcolemmal Ca²⁺ pump - sarcolemmal Na⁺–Ca²⁺ exchange - membrane phospholipids - cardiac atrophy - Ca²⁺ transport in myocardium - transplanted heart

Full Text

References

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