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Thromb Haemost 1979; 42(02): 743-751
DOI: 10.1055/s-0038-1666912
Original Article
Schattauer GmbH Stuttgart

Effects of Tsetse (Glossina morsitans morsitans Westw.) (Diptera: Glossinidae) Salivary Gland Homogenate on Coagulation and Fibrinolysis

Kenneth R Parker
The Departments of Entomology and Medicine, University of Alberta, Edmonton, Alberta, Canada
,
Michael J Mant
The Departments of Entomology and Medicine, University of Alberta, Edmonton, Alberta, Canada
› Author Affiliations
Further Information

Publication History

Received 01 June 1978

Accepted 30 September 1978

Publication Date:
23 August 2018 (online)

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Summary

The saliva of the tsetse, Glossina morsitans morsitans Westwood, has antithrombin anticoagulant activity and inhibits thrombin’s esterolytic activity. It has no other detectable anticoagulant properties. The anticoagulant elutes in a single peak on Sephadex fraction, is immediately acting, heat and storage stable, and has a molecular weight of 11-13,000. Unlike heparin it is not neutralized by protamine sulphate or toluidine blue and does not require the co-factor, antithrombin III, for optimal anticoagulant activity. It has similar properties to hirudin, but does not elute with a protein peak upon Sephadex fractionation and has a slightly different molecular weight. Salivary gland homogenates contained neither a plasminogen activator nor fibrinolytic activity. The sera of rabbits used to maintain tsetses, which contained precipitating antibodies against saliva, did not neutralize the salivary anticoagulant in vitro. The properties of this anticoagulant suggest that it might be a potentially useful antithrombotic agent in man.

 

  • References

  • 1 Bagdy D, Barabás E, Gräf L. 1973; Large scale preparation of hirudin. Thrombosis Research 2: 229
    CrossrefPubMedGoogle Scholar
  • 2 Barker CF, Rosato FE, Roberts B. 1966; Peripheral arterial embolism. Surgery Gynecology and Obstetrics 123: 22
    PubMedGoogle Scholar
  • 3 Bell WR, Tomasulo PA, Alving BM, Duffy TP. 1976; Thrombocytopenia occuring during the administration of heparin. Annals of Internal Medicine 85: 155
    CrossrefPubMedGoogle Scholar
  • 4 Bell WR, Anderson ND, Anderson AO. 1977; Heparin-induced coagulopathy. Journal of Laboratory and Clinical Medicine 89: 741
    PubMedGoogle Scholar
  • 5 Bishop R, Ekert H, Gilchrist G, Shanbrom E, Fekete L. 1970; The preparation and evaluation of a standardized fibrin plate for the assessment of fibrinolytic activity. Thrombosis Diathesis Haemorrhagica 23: 202
    PubMedGoogle Scholar
  • 6 Blix S. 1961; Studies on the fibrinolytic system in the euglobulin fraction of human plasma. Scandinavian Journal of Clinical and Laboratory Investigation Supplement 58: 3
    PubMedGoogle Scholar
  • 7 Cornwall JW, Patton WS. 1914; Some observations on the salivary secretions of the commoner bloodsucking insects and ticks. Indian Journal of Medical Research 2: 569
    PubMedGoogle Scholar
  • 8 Dacie JV, Lewis SM. 1968. Practical Hematology. 4. ed J A. Churchill; London:
    Google Scholar
  • 9 Eika C. 1972; The platelet aggregating effect of eight commercial heparins. Scandinavian Journal of Haematology 9: 480
    PubMedGoogle Scholar
  • 10 Fagerhol MK, Abildgaard U. 1970; Immunological studies on human Antithrombin III. Scandinavian Journal of Haematology 7: 10
    PubMedGoogle Scholar
  • 11 Fairbairn H, Williamson J. 1956; The composition of tsetse-fly saliva. I. A histochemical analysis. Annals of Tropical medicine and Parasitology 50: 322
    CrossrefPubMedGoogle Scholar
  • 12 Gooding RH. 1972; Digestive processes of haematophagous insects. I. A literature review. Quaestiones entomologicae 8: 5
    PubMedGoogle Scholar
  • 13 Gooding RH, Rolseth BM. 1976 Digestive processes of haematophagous insects. IX. Partial purification and some properties of six proteolytic enzymes from the tsetse fly Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Canadian Journal of Zoology. 54. 1950
    PubMedGoogle Scholar
  • 14 Hawkins RI. 1966; Factors affecting blood clotting from salivary glands and crop of Glossina austeni. Nature (London) 212: 738
    CrossrefPubMedGoogle Scholar
  • 15 Hellmann K, Hawkins RI. 1965; Prolixin-S and Prolixin-G; two anticoagulants from Rhodnius prolixus Stäl. Nature (London) 207: 265
    CrossrefPubMedGoogle Scholar
  • 16 Hellmann K, Hawkins RI. 1967; The action of the tick extracts on blood coagulation and fibrinolysis. Thrombosis Diathesis Haemorrhagica 18: 611
    PubMedGoogle Scholar
  • 17 Klein LA, Hall RL, Smith RB. 1972; Surgical treatment of priapism: with a note on heparin-induced priapism. Journal of Urology 108: 104
    CrossrefPubMedGoogle Scholar
  • 18 Latallo ZS, Teisseyre E. 1971; Evaluation of Reptilase R and thrombin clotting time in the presence of fibrinogen degradation products and heparin. Scandinavian Journal of Haematology Supplement 13: 261
    PubMedGoogle Scholar
  • 19 Lester HM O, Lloyd L. 1928; Notes on the process of digestion in tsetse flies. Bulletin of entomological Research 19: 39
    CrossrefPubMedGoogle Scholar
  • 20 Mant MJ, Hirsh J, Gauldie J, Bienenstock J, Pineo GF, Luke KH. 1973; Von Willebrand’s syndrome presenting as an acquired bleeding disorder in association with a monoclonal gammopathy. Blood 42: 429
    PubMedGoogle Scholar
  • 21 Marciniak E, Gockerman JP. 1977; Heparin-induced decrease in circulating antithrombin-III. Lancet 2: 581
    PubMedGoogle Scholar
  • 22 Margolis J, Bruce L. 1964; An experimental approach to the kinetics of blood coagulation. British Journal of Haematology 10: 513
    CrossrefPubMedGoogle Scholar
  • 23 Markwardt F. 1961; Der Einfluß von Thrombininhibitoren auf “Nebenwirkungen” des Thrombins. (English summary). Thrombosis Diathesis Haemorrhagica 5: 576
    PubMedGoogle Scholar
  • 24 Mustard JF, Murphy EA, Downie HG, Rowsell HC. 1963; Heparin and thrombus formation: Early suppression and late enhancement. British Journal of Haematology 9: 548
    CrossrefPubMedGoogle Scholar
  • 25 Seegers WH. 1967. Blood clotting enzymology. Academic Press; New York:
    Google Scholar
  • 26 Sherry, Sol. Troll W. 1954; The action of thrombin on synthetic substrates. Journal of biological Chemistry 95: 208
    PubMedGoogle Scholar
  • 27 Tertrin C, De La Llosa P, Junsz M. 1966; Étude de quelques constantes physico-chimiques de l’hirudine. Biochimica et Biophysica Acta 124: 380
    CrossrefPubMedGoogle Scholar
  • 28 Thorson RE. 1956; The effect of the amphidial glands, excretory glands, esophagus of adults of Ancylostoma caninum on the coagulation of dog’s blood. Journal of Parasitology 42: 26
    CrossrefPubMedGoogle Scholar