A Rapid One-Step Immunoradiometric Assay for Factor VIII-Procoagulant Antigen Utilizing Monoclonal Antibodies

H V Stel; E C I Veerman; J G Huisman; M C Janssen; J A van Mourik

doi:10.1055/s-0038-1665329

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1983; 50(04): 860-863
DOI: 10.1055/s-0038-1665329

Original Article

Schattauer GmbH Stuttgart

A Rapid One-Step Immunoradiometric Assay for Factor VIII-Procoagulant Antigen Utilizing Monoclonal Antibodies

H V Stel

The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands

,

E C I Veerman

The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands

,

J G Huisman

The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands

,

M C Janssen

The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands

,

J A van Mourik

The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands

› Author Affiliations

Abstract

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Summary

A two-site immunoradiometric assay for factor VIII-procoagulant antigen (VIIICAg) that relies completely on monoclonal antibodies has been developed. By selecting an appropriate combination of these antibodies, it was possible to develop an assay in which the radiolabelled monoclonal antibody did not inhibit the binding of antigen to the solid-phase monoclonal antibodies. Thus, the entire test could be carried out as a one-step procedure. With this one-step assay, an amount of 0.0025 U VIIICAg/ml plasma could be detected after 4 hr of incubation, whereas 18 hr of incubation resulted in a lower limit of sensitivity of 0.0005 U VIIICAg/ml. The use of a one-step assay provides a significant advantage over the conventional two-step assay by simplifying, shortening and rendering the performance of the assay more convenient.

Keywords

Monoclonal antibodies - One-step IRMA - VIIICAg - Haemophilia A

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References

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