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Thromb Haemost 1983; 50(04): 835-837
DOI: 10.1055/s-0038-1665323
Original Article
Schattauer GmbH Stuttgart

Cell-Free Synthesis of Factor VIII Related Protein

Vivian Chan
The Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong
,
T K Chan
The Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong
› Author Affiliations
Further Information

Publication History

Received 26 May 1983

Accepted 27 September 1983

Publication Date:
18 July 2018 (online)

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Summary

Polyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunopredpitation, the translation product was found to contain 1.58% VIH related protein (VIIIR), which has a molecular weight of 0.26 × 106, similar to the subunit of VIIIR present in normal plasma. This indicated that VIIIR messenger RNA is responsible for the synthesis of subunits only and intracellular post-translational events are required for the assembly of tetramer and multimers.

Keywords

Factor VIII related protein - Cell-free synthesis - Human endothelial cells - Messenger RNA
 

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