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Thromb Haemost 1983; 50(03): 639-644
DOI: 10.1055/s-0038-1665276
Original Article
Schattauer GmbH Stuttgart

New “Ex Vivo” Radioisotopic Method of Quantitation of Platelet Deposition – Studies in Four Animal Species

L Badimon
1   The Division of Cardiovascular Diseases and Internal Medicine, Mayo Clinic, Rochester, MN, and Mount Sinai Medical Center, New York, NY, U.S.A.
2   The Section of Diagnostic Nuclear Medicine, Mayo Clinic, Rochester, MN, and Mount Sinai Medical Center, New York, NY, U.S.A.
3   The Thrombosis and Atherosclerosis Unit, Barcelona, Spain
,
V Fuster
1   The Division of Cardiovascular Diseases and Internal Medicine, Mayo Clinic, Rochester, MN, and Mount Sinai Medical Center, New York, NY, U.S.A.
,
J H Chesebro
1   The Division of Cardiovascular Diseases and Internal Medicine, Mayo Clinic, Rochester, MN, and Mount Sinai Medical Center, New York, NY, U.S.A.
,
M K Dewanjee
2   The Section of Diagnostic Nuclear Medicine, Mayo Clinic, Rochester, MN, and Mount Sinai Medical Center, New York, NY, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 30 August 1982

Accepted 16 June 1983

Publication Date:
18 July 2018 (online)

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Summary

We have developed a sensitive and quantitative method of “ex vivo” evaluation of platelet deposition on collagen strips, from rabbit Achilles tendon, superfused by flowing blood and applied it to four animal species, cat, rabbit, dog and pig. Autologous platelets were labeled with indium-111-tropolone, injected to the animal 24 hr before the superfusion and the number of deposited platelets was quantitated from the tendon gamma-radiation and the blood platelet count.

We detected some platelet consumption with superfusion time when blood was reinfused entering the contralateral jugular vein after collagen contact but not if blood was discarded after the contact. Therefore, in order to have a more physiological animal model we decided to discard blood after superfusion of the tendon.

In all species except for the cat there was a linear relationship between increase of platelet on the tendon and time of exposure to blood superfusion. The highest number of platelets deposited on the collagen was found in cats, the lowest in dogs.

Ultrastructural analysis showed the platelets were deposited as aggregates after only 5 min of superfusion.

Keywords

Platelet deposition - Platelet labeling - Platelet activation
 

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