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Thromb Haemost 1986; 56(03): 376-381
DOI: 10.1055/s-0038-1661686
Original Article
Schattauer GmbH Stuttgart

Role of Red Blood Cells in the Early Stages of Platelet Activation by Collagen

M Teresa Santos
*   The Research Center, Hospital ⟫La Fe⟪, Valencia, Spain
,
Juana Vallés
*   The Research Center, Hospital ⟫La Fe⟪, Valencia, Spain
,
Justo Aznar
**   The Department of Clinical Pathology, Hospital ⟫La Fe⟪, Valencia, Spain
,
J Luis Pérez-Requejo
**   The University of Carabobo, Valencia, Venezuela
› Author Affiliations
Further Information

Publication History

Received 21 May 1986

Accepted after revision 19 September 1986

Publication Date:
18 July 2018 (online)

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Summary

Red blood cells (RBC) increase the proaggregatory capacity of a cell-free supernatant obtained by stimulating platelet-rich plasma (PRP) samples with collagen (1 μg/ml) as measured by the BASIC wave; this effect increases with the number of RBC and is proportionally greater with a lower number of platelets or when lower collagen concentrations are used.

Aspirin (ASA) modifies the RBC behaviour in relation to their platelet-collagen interaction. This is demonstrated by the fact that when PRP and RBC obtained from the same subjects before and two hours after the ingestion of ASA (0.5 g) were mixed, it was found that non-AS A-RBC stimulate ASA-PRP, probably through a platelet cyclooxygenase independent pathway; ASA-RBC, however, stimulate non-ASA-PRP, but not ASA-PRP, which suggests that they may need an active platelet cyclooxygenase system for their action. This effect of ASA on RBC is not transient and was also observable 48 h after ASA ingestion. In addition, it was found that ASA-RBC greatly increase the activation of a mixture containing a small proportion of non-ASA-PRP in ASA-PRP, a situation that is expected to be encountered “in vivo” after ASA treatment. This effect of ASA-RBC on platelet activation may help to explain the sometimes contradictory clinical effect of aspirin as an antithrombotic drug.

Key words

Platelet activation - Erythrocytes - Platelet - Erythrocyte interactions - Aspirin
 

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