Thromb Haemost 1986; 56(01): 063-065
DOI: 10.1055/s-0038-1661604
Original Article
Schattauer GmbH Stuttgart

Cellular Localisation in Placenta of Placental Type Plasminogen Activator Inhibitor

B Åstedt
*   The Research Laboratory of the Department of Obstetrics and Gynaecology, University Hospital, Lund, Sweden
,
I Hägerstrand
The Department of Pathology, University Hospital, Lund, Sweden
,
I Lecander
*   The Research Laboratory of the Department of Obstetrics and Gynaecology, University Hospital, Lund, Sweden
› Author Affiliations
Further Information

Publication History

Received 07 January 1986

Accepted after revision 26 May 1986

Publication Date:
13 July 2018 (online)

Summary

A specific plasminogen activator inhibitor is known to occur in placenta and in pregnancy plasma. Immunohistochemical methods with polyclonal and monoclonal antibodies against the inhibitor were used for its localisation in term placentas. Immunoreactive material was found in the trophoblastic epithelium. It was absent in the stroma of the chorion villi.

 
  • References

  • 1 Åstedt B, Pandolfi M. On release and synthesis of fibrinolytic activators in human organ culture. Eur J Clin Biol Res 1972; 17: 261-267
  • 2 Levin EG, Loskutoff DJ. Comparative studies of the fibrinolytic activity of cultured vascular cells. Thromb Res 1979; 15: 869-878
  • 3 Rijken DC, Wijngaards G, Welbergen J. Relationship between tissue plasminogen activator and the activators in blood and vascular wall. Thromb Res 1980; 18: 815-830
  • 4 Astrup T, Permin PM. Fibrinolysis in the animal organism. Nature 1947; 159: 681-682
  • 5 Husain SS, Gurewich V, Lipinski B. Purification of a new high molecular weight form of urokinase from urine. Thromb Haemostas 1981; 46: 11 (Abstr.)
  • 6 Wun TC, Ossowski L, Reich E. A proenzyme form of human urokinase. J Biol Chem 1982; 257: 7262-7268
  • 7 Husain SS, Gurewich V, Lipinski B. Purification and partial characterization of a single-chain, high molecular weight form of urokinase from human urine. Arch Biochem Biophys 1983; 220: 31-38
  • 8 Larsson Å, Åstedt B. Immunohistochemical localisation of tissue plasminogen activator and urokinase in the vessel wall. J Clin Pathol 1985; 38: 140-145
  • 9 Skriver L, Larsson LI, Kielberg V, Nielsen LS, Andresen PB, Kristensen P, Dano K. Immunocytochemical localization of urokinase-type plasminogen activator in Lewis lung carcinoma. J Cell Biol 1984; 99: 753-758
  • 10 Camiolo SM, Markus G, Englander LS, Siuta MR, Hobika GH, Kohga S. Plasminogen activator content and secretion in explants of neoplastic and benign human prostate tissues. Cancer Res 1984; 44: 311-318
  • 11 Collen D. Report of the meeting of the subcommittee on fibrinolysis, San Diego, CA, USA, July 13, 1985. Thromb Haemostas 1985; 54: 893
  • 12 Scott RW, Bergman BL, Bajpai A, Hersh RT, Rodriques H, Jones BN, Barreda C, Watts S, Baker JB. Protease Nexin. J Biol Chem 1985; 260: 7029-7034
  • 13 Chmielewska J, Rånby M, Wiman B. Evidence for a rapid inhibitor to tissue plasminogen activator in plasma. Thromb Res 1983; 31: 427-436
  • 14 Loskutoff DJ, Mussoni L. Interactions between fibrin and the plasminogen activators produced by cultured endothelial cells. Blood 1983; 62: 62-68
  • 15 Juhan-Vague I, Moerman B, de Cock F, Aillaud MF, Collen D. Plasma levels of a specific inhibitor of tissue-type plasminogen activator (and urokinase) in normal and pathological conditions. Thromb Res 1984; 33: 523-530
  • 16 Thorsen S, Philips M. Isolation of tissue-type plasminogen activator-inhibitor complexes from human plasma. Evidence for a rapid plasminogen activator inhibitor. Biochim Biophys Acta 1984; 802: 111-118
  • 17 Philips M, Juul AG, Thorsen S. Human endothelial cells produce a plasminogen activator inhibitor and a tissue-type plasminogen activator-inhibitor complex. Biochim Biophys Acta 802: 99-110
  • 18 Wiman B, Chmielewska J, Rånby M. Inactivation of tissue plasminogen activator in plasma. Demonstration of a complex with a new rapid inhibitor. J Biol Chem 1984; 259: 3644-3646
  • 19 Kawano T, Morimoto K, Uemura Y. Urokinase inhibitor in human placenta. Nature 1968; 217: 253-254
  • 20 Åstedt B, Pandolfi M, Nilsson IM. Inhibitory effect of placenta on plasminogen activation in human organ culture. Proc Soc Exp Biol Med 1972; 139: 1421-1424
  • 21 Åstedt B. Demonstration of significance of placenta in depression of fibrinolytic activity during pregnancy. J Obst Gyn Br Commonw 1972; 79: 205-206
  • 22 Åstedt B, Lecander I, Brodin T, Lundblad A, Löw K. Purification of a specific placental plasminogen activator inhibitor by monoclonal antibody and its complex formation with plasminogen activator. Thromb Haemostas 1985; 53: 122-125
  • 23 Lecander I, Åstedt B. Isolation of a new specific plasminogen activator inhibitor from pregnancy plasma. Br J Haematol 1986; 221-228
  • 24 Taylor CR. Immunoperoxidase techniques: practical and theoretical aspects. Arch Pathol Lab Med 1978; 102: 113-121
  • 25 Sheppard BL, Bonnar J. Fibrinolysis in decidual spiral arteries in late pregnancy. Thromb Haemostas 1978; 39: 751-758
  • 26 Todd AS. Histological localization of fibrinolysin activator. J Path Bact 1959; 78: 281-283
  • 27 Chapman Jr HA, Stone OL. Characterization of a macrophage-derived plasminogen-activator inhibitor. Biochem J 1985; 230: 109-116
  • 28 Nilsson IM, Ljungnér H, Tengborn L. Two different mechanisms in patients with venous thrombosis and defective fibrinolysis: low concentration of plasminogen activator or increased concentration of plasminogen activator inhibitor. Br Med J (Clin Res) 1985; 290: 1453-1456