Identification of t-PA as the Major Active Plasminogen Activator in Human Milk

J M Marshall; M C P Rees; S A Cederholm-Williams

doi:10.1055/s-0038-1661537

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1986; 55(02): 279-281
DOI: 10.1055/s-0038-1661537

Original Article

Schattauer GmbH Stuttgart

Identification of t-PA as the Major Active Plasminogen Activator in Human Milk

Authors

J M Marshall

The Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK
M C P Rees

The Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK
S A Cederholm-Williams

The Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK

Abstract

PDF Download

Summary

Human colostrum and milk contain high levels of plasminogen activator when compared with blood. These levels are inversely related to the duration of lactation and the activity appears to be due to tissue type plasminogen activator present in multiple (65-190 Kd) molecular weight forms.

Key words

Tissue plasminogen activator - Human milk

PDF (1092 kb)

References

References
1 Astrup T, Stemdorff I. A fibrinolytic system in human milk. Pro Soc Exp Biol Med 1953; 84: 605-609
2 Horie N, Okamoto U, Ogawa C. Studies on the plasminogen activating system in human milk. VI. Differences in properties between milk activator and glandular kallikrein. Nippon Ketsueki Gakki Zasshi 1982; 45: 1099-1106
3 Okamoto U, Horie N, Nagamatsu Y, Yamamoto J. Distribution of plasminogen activator in human milk samples collected at various days after delivery. Acta Haematol Jap 1980; 43: 743-746
4 Okamoto U, Horie N, Nagamatsu Y, Yamamoto JI. Plasminogen activator in human early milk: its partial purification and characterisation. Thromb Haemostas 1981; 45: 121-126
5 Reiner GC, Katzenellenbogen BS, Bindal RD, Katzellenbogen JA. Biological activity and receptor binding activity of a strongly interacting estrogen in human breast cancer cells. Cancer Research 1984; 44: 2302-2308
6 Huff KK, Lippman ME. Hormonal control of plasminogen activator secretion in ZR 75-1 human breast cancer cells in culture. Endocrinology 1984; 114: 1702-1710
7 Isotalo H, Tryggvason K, Vierikko P, Kauppila A, Vihko R. Plasminogen activators and steroid receptor concentrations in normal, benign, and malignant breast and ovarian tissue. Anticancer Res 1983; 3: 331-335
8 Butler WB, Kirkland WL, Gargala TL, Goran N, Kelsey WH, Berlinski PJ. Steroid stimulation of plasminogen activator production in a human breast cancer cell line (MCF-7). Cancer Res 1983; 43: 1637-1641
9 Reich E. Activation of plasminogen: a general mechanism for producing extracellular proteolysis. In: Molecular basis of biological degradative processes Berlin RD. et al (eds) Academic Press; New York: 1977: 155-169
10 Evers JL, Patel J, Madeja JM, Schneider SL, Hobika GH, Camiolo SM, Markus G. Plasminogen activator activity and composition in human breast cancer. Cancer Research 1982; 42: 219-226
11 Astrup T, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 1952; 1940: 346-351
12 Granelli-Piperno A, Reich E. A study of proteases and protease-inhibitor complexes in biological fluids. J Exp Med 1978; 148: 223-234
13 Verheijen JH, Chang GT, Kluft C. Evidence for the occurrence of a fast acting inhibitor for tissue type plasminogen activator in human plasma. Thromb Haemostas 1984; 51: 392-395
14 Thorsen S, Philips M. Isolation of tissue type plasminogen activator inhibitor complexes from human plasma: Evidence for a rapid plasminogen activator inhibitor. Biochim Biophys Acta 1984; 802: 111-118
15 Cederholm-Williams SA. Molecular biology of plasminogen activators and recombinant DNA technology. BioEssays 1984; 1: 168-173