Thromb Haemost 1986; 55(01): 098-103
DOI: 10.1055/s-0038-1661456
Original Article
Schattauer GmbH Stuttgart

Triton X-114 Phase Separation of Platelet Membrane Glycoproteins from Normal Subjects and a Patient with Type I Thrombasthenia

U Khanduri
The Department of Medicine, St. Vincent’s Hospital, Fitzroy, Melbourne, Australia
,
S Clark
The Department of Medicine, St. Vincent’s Hospital, Fitzroy, Melbourne, Australia
,
I D Walker
*   The Department of Pathology, University of Melbourne, Parkville, Melbourne, Australia
,
K G Chamberlain
The Department of Medicine, St. Vincent’s Hospital, Fitzroy, Melbourne, Australia
,
D G Penington
The Department of Medicine, St. Vincent’s Hospital, Fitzroy, Melbourne, Australia
› Author Affiliations
Further Information

Publication History

Received 05 August 1985

Accepted 14 November 1985

Publication Date:
19 July 2018 (online)

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Summary

Surface-labelled normal and thrombasthenic platelets have been subjected to phase separation in Triton X-114. Triton-rich and Triton-poor fractions have been analysed by SDS-PAGE and IEF-SDS-PAGE. Partitioning characteristics of the major glycoproteins have been defined. The Triton-rich fraction contained GPIIb, III, IV, VI, VII, VIII, GP38 and the IIb β subunit. In contrast, the Triton-poor fraction contained the HMWGP, GPIa, Ib, IIb, III, V and GPIX.

Analysis of the platelet membrane glycoproteins of a patient with Type 1 thrombasthenia has been carried out using Triton X-114. The value of the method in diagnosis of this condition and differences between our findings and those published previously are discussed.