Nomenclature of Secreted Platelet Proteins – Report of the Working Party on Secreted Platelet Proteins of the Subcommittee on Platelets

Karen L Kaplan; Stefan Niewiarowski

doi:10.1055/s-0038-1661294

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 53(02): 282-284
DOI: 10.1055/s-0038-1661294

International Committee Communications

Schattauer GmbH Stuttgart

Nomenclature of Secreted Platelet Proteins – Report of the Working Party on Secreted Platelet Proteins of the Subcommittee on Platelets

Karen L Kaplan

¹The Department of Medicine, Columbia University College of Physicians & Surgeons, NY, NY, USA

,

Stefan Niewiarowski

²The Specialized Center of Research in Thrombosis, Temple University Health Sciences Center, Philadelphia, PA, USA

› Author Affiliations

Abstract

PDF Download

Summary

Standard nomenclature for a number of secreted platelet proteins was agreed upon by The Working Party on Secreted Platelet Proteins of the Subcommittee on Platelets. Platelet factor 4 will continue to be used for the molecule with high heparin affinity, subunit molecular weight of 7780, and the described amino acid sequence. β-Thromboglobulin will be used to designate β-Thromboglobulin (81 amino acids/subunit, β-mobility on cellulose-acetate electrophoresis, pI 7), low-affinity platelet factor 4 (85 amino acids/subunit, y-mobility on cellulose-acetate electrophoresis, pI 8), and platelet basic protein (94 amino acids/ subunit, pI 10) when these are measured immunologically in plasma, but that thromboglobulin with a superscript designation of the pI should be used when assays are conducted on samples after isoelectric focusing, and a subscript amino-terminal amino acid can be added when a purified protein is described. Thrombospondin will continue to be the designation for the high molecular weight trimer that has previously been called thrombospondin or glycoprotein G. Platelet derived growth factor will be used for the group of closely related proteins of molecular weight about 30,000 and isoelectric point about 10.

Keywords

Platelet factor 4 - β-Thromboglobulin - Low-affinity platelet factor 4 - Platelet basic protein - Retroperitoneal bleeding - Thrombospondin - Platelet-derived growth factor

PDF (142 kb)

References

References
1 Deuel TF, Keim PS, Fanner M, Heinrikson RL. The complete amino acid sequence of human platelet factor 4. Proc Natl Acad Sci USA 1981; 74: 2256-2258
2 Hermodsen M, Schmer G, Kurachi K. Isolation, crystallization and primary amino acid sequence of human platelet factor 4. J Biol Chem 1977; 252: 6276-6279
3 Morgan FJ, Begg GS, Chesterman CN. Primary structure of human platelet factor 4. Thromb Haemostas 1977; 38: 231 (Abstr.)
4 Walz DA, Wu VY, deLamo R, Dene H, McCoy LE. Primary structure of human platelet factor 4. Thromb Res 1977; 11: 893-898
5 Kaser-Glanzmann R, Jakabova M, Luscher EF. Isolation and some properties of the heparin-neutralizing factor (PF4) released from human blood platelets. Experientia 1972; 28: 1221-1223
6 Moore S, Pepper DS, Cash JD. Platelet anti-heparin activity: The isolation and characterization of platelet factor 4 released from thrombin-aggregated washed human platelets and its dissociation into subunits and the isolation of membrane- bound anti-heparin activity. Biochim Biophys Acta 1975; 379: 370-384
7 Ginsberg MM, Hopkins R, Sigrist P, Painter R. Purification of heparin neutralizing protein from rabbit platelets and its homology with human platelet factor 4. J Biol Chem 1979; 254: 12365-12371
8 Ciaglowski RE, Snow JW, Walz DE. Bovine platelet antiheparin protein: platelet factor 4. Ann NY Acad Sci 1981; 370: 668-679
9 Rucinski B, Poggi A, James P, Holt JC, Niewiarowski S. Purification of two heparin-binding proteins from porcine platelets and their homology with human secreted platelet proteins. Blood 1983; 61: 1072-1080
10 Moore S, Pepper DS, Cash JD. The isolation and characterization of a platelet- specific β-globulin (β-thromboglobulin) and the detection of antiurokinase and antiplasmin released from thrombin-aggregated washed human platelets. Biochim Biophys Acta 1975; 379: 360-369
11 Moore S, Pepper DS. In Platelets in Biology and Pathology. Gordon JL. (Ed) Elsevier; North Holland, Amsterdam: 1976. p 293
12 Begg GS, Pepper DS, Chesterman CN, Morgan FJ. Complete covalent structure of human p-thromboglobulin. Biochemistry 1978; 17: 1739-1744
13 Rucinski B, Niewiarowski S, James P, Walz DA, Budzynski AZ. Antiheparin proteins secreted by human platelets: purification, characterization, and radioimmunoassay. Blood 1979; 53: 47-62
14 Niewiarowski S, Walz DA, James P, Rucinski B, Kueppers F. Identification and separation of secreted platelet proteins by isoelectric focusing. Evidence that low-affinity platelet factor 4 is converted to β-thromboglobulin by limited proteolysis Blood 1980; 55: 453-456
15 Holt JC, Harris ME, Lange E, Rucinski B, Henschen A, Niewiarowski S. Platelet basic protein (PBP) is the precursor of low affinity platelet factor 4 (LA- PF4) and p thromboglobin (PTG). Fed Proc 1982; 41: 528
16 Paul D, Niewiarowski S, Vanma KG, Rucinski B, Rucker S, Lange E. Human platelet basic protein associated with antiheparin and antigenic activities. Purification and partial characterisation Proc Natl Acad Sci (USA) 1980; 77: 5914-5918
17 Hagen I. Effects of thrombin on washed human platelets: changes in subcellular fractions. Biochim Biophys Acta 1975; 392: 242-254
18 Hagen I, Olsen T, Solum NO. Studies on subcellular fractions of human platelets by the lactoperoxidase - iodination technique. Biochim Biophys Acta 1976; 455: 214-225
19 Lawler JW, Chao FC, Fang PH. Observation of a high molecular weight platelet protein released by thrombin. Thromb Haemostas 1977; 37: 355-357
20 Lawler JW, Slayter HS, Coligan JE. Isolation and characterization of a high molecular weight glycoprotein from human blood platelets. J Biol Chem 1978; 253: 8609-8616
21 Mosher DF, Jaffe EA. Synthesis and secretion of thrombospondin by cultured human endothelial cells. J Cell Biol 1982; 93: 343-348
22 McPherson J, Sage IT, Bornstein P. Isolation and characterization of a glycoprotein secreted by aortic endothelial cells in culture. Apparent identity with platelet thrombospondin J Biol Chem 1981; 256: 11330-11336
23 Phillips DR, Jennings LK, Prasanna HR. Ca⁺⁺ mediated association of glycoprotein G (thrombin sensitive protein, thrombospondin) with human platelets. J Biol Chem 1980; 155: 11629-11632
24 Margossian SS, Lawler JW, Slayter H. Physical characterization of platelet thrombospondin. J Biol Chem 1981; 256: 7495-7500
25 Lawler J, Chao FC, Cohen CM. Evidence for calcium-sensitive structure in platelet thrombospondin: Isolation and partial characterization of thrombospondin in the presence of calcium. J Biol Chem 1982; 257: 12257-12265
26 Baenziger NL, Brodie GN, Majerus PW. A thrombin-sensitive protein of human platelet membranes. Proc Natl Acad Sci USA 1971; 68: 240-243
27 Baenziger NL, Brodie GN, Majerus PW. Isolation and properties of a thrombin-sensitive protein of human platelets. J Biol Chem 1972; 247: 2723-2731
28 Heldin C-H, Westermark B, Wasteson A. Platelet-derived growth factor: purification and partial characterization. Proc Natl Acad Sci 1979; 76: 3722-3726
29 Antoniades HN, Scher CD, Stiles CD. Purification of human platelet-derived growth factor. Proc Natl Acad Sci USA 1979; 76: 1809-1813
30 Raines EW, Ross RPlatelet-derived. Platelet-derived growth factor: I. High yield purification and evidence for multiple forms. J Biol Chem 1982; 257: 5154-5160
31 Witte LD, Kaplan KL, Nossel HL, Lages BA, Weiss HJ, Goodman DeWS. Studies of the release from human platelets of the growth factor for cultured human arterial smooth muscle cells. Circ Res 1978; 42: 402-409
32 Habenicht Jr A, Glomset JA, Ross R. Relation of cholesterol and mevalonic acid to the cell cycle in smooth muscle and Swiss 3T3 cells stimulated to divide by platelet-derived growth factor. J Biol Chem 1980; 255: 5234-5240
33 Witte LD, Cornicelli JA, Miller RW, Goodman DeWS. Effects of platelet derived and endothelial cell-derived growth factors on the low density lipoprotein receptor pathway in cultured human fibroblasts. J Biol Chem 1982; 257: 5392-5401
34 Deuel TF, Huang SS, Huang JS. PDGF stimulated protein kinase activity in human smooth muscle cells. Thromb Haemostas 1983; 50: 71 (Abstr.)
35 Williams LT, Frackelton Jr AR. Platelet-derived growth factor activation of tyrosine-kinase in vascular smooth muscle: detection by monoclonal antibody to phosphotyrosine. Circulation 1983; (Suppl. 68) (SuppIII): 73
36 Deuel TF, Senior RM, Huang JS, Griffin GL. Chemotaxis of monocytes and neutrophils to platelet-derived growth factor. J Clin Invest 1982; 69: 1046-1049
37 Senior RM, Griffin GL, Huang JS, Walz DA, Deuel TF. Chemotactic activity of alpha-granule proteins for fibroblasts. J Cell Biol 1983; 96: 382-385
38 Grotendorst GR, Seppa HE, Kleinman HK, Martin GR. Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc Natl Acad Sci 1981; 78: 3669-3672
39 Seppa H, Grotendorst GR, Seppa S, Schiffmann E, Martin GR. Platelet- derived growth factor is chemotactic for fibroblasts. J Cell Biol 1982; 92: 584-588
40 Antoniades HN, Hunkapiller MW. Human platelet-derived growth factor: Amino-terminal amino acid sequence. Science 1983; 220: 963-965
41 Waterfield MD, Scrace GT, Whittle N, Stroobant P, Johnsson A, Wasteson A, Westermark B, Heldin C, Huang JS, Deuel F. Platelet-derived growth factor is structurally related to the putative transforming protein p28 of simian sarcoma virus. Nature 1983; 304: 35-39
42 Heldin CH, Wasteson A, Westermark B. Partial purification and characterization of platelet factors stimulating the multiplication of normal human glial cells. Exp Cell Res 1977; 109: 429-437
43 Kepner N, Creasy G, Lipton A. Platelets as a source of cell-proliferatory activity in platelets: A Multidisciplinary Approach. de Gaetano G, Garattini S. (Eds) Raven Press; New York: 1978. p 205
44 Assoian RK, Komoriya A, Meyers CA, Miller DM, Spora MB. Transforming growth factor-B in human platelets. J Biol Chem 1983; 258: 7155-7160
45 Clemmons DR, Isley WL, Brown MT. Dialyzable factor in human serum of platelet origin stimulates endothelial cell replication and growth. Proc Natl Acad Sci 1983; 80: 1641-1645
46 Castor CW, Ritchie JC, Scott MF, Whitney SL. Connective tissue activation. XI Stimulation of glycosaminoglycans and DNA formation by a platelet factor Arthritis Rheum 1977; 20: 859-868
47 Castor CW, Ritchie JC, Williams CH, Scott ME, Whitney SL, Meyers SL, Sloan TB, Anderson BE. Connective tissue activation XIV Composition and action of human platelet autocoid mediator. Arthritis Rheum 1979; 22: 260-272
48 Castor CW, Miller JW, Walz DA. Structural and biological characteristics of connective tissue activating peptide, a major human platelet-derived growth factor. Proc Natl Acad Sci USA 1983; 80: 765-769
49 Niewiarowski S, Paul D. Platelet granule proteins with mitogenic and antiheparin activity. In Platelets in Biology and Pathology 2. Gordon IL. (Ed) Elsevier; North Holland: 1981. pp 91-106