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Thromb Haemost 1985; 53(02): 264-267
DOI: 10.1055/s-0038-1661290
Original Article
Schattauer GmbH Stuttgart

Incorporation of Some Eicosaenoic Acids into Endothelial Cells – Effect on Platelet Inhibitory Activity and Prostacyclin Production

Béatrice Sicard
The Institut Pasteur, INSERM U.63 and Laboratoire d’Hémobiologie, Faculté de Médecine Alexis Carrel, Lyon, France
,
Michel Lagarde
The Institut Pasteur, INSERM U.63 and Laboratoire d’Hémobiologie, Faculté de Médecine Alexis Carrel, Lyon, France
› Author Affiliations
Further Information

Publication History

Received 09 November 1984

Accepted 05 February 1985

Publication Date:
18 July 2018 (online)

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Summary

Primary cultures of endothelial cells from human umbilical veins were grown until confluency. Then, dihomogammalinolenic acid (DHLA or 20:3n-6) and eicosapentaenoic acid (EPA or 20:5n-3), precursors of monoenoic and trienoic prostanoids, respectively, as well as 5,8,11-eicosatrienoic acid (20:3n-9), and isomer of DHLA, were incorporated into endothelial lipids. DHLA-rich endothelial cells had a decreased capacity of prostacyclin production. By contrast EPA- or 20:3n-9-rich endothelial cells were comparable to controls in this respect. DHLA and EPA were efficiently acylated into cell phospholipids and triglycerides at the opposite of 20:3n-9. It is suggested that both DHLA and EPA could alter the liberation of endogenous arachidonic acid for prostacyclin synthesis but this might be counterbalanced in EPA-rich endothelial cells by PGI3 production. We conclude that DHLA enrichment of endothelial cell lipids may impair the possible beneficial effect of the acid upon platelet functions whereas that of EPA would not be modified.

Keywords

Endothelial cells - Prostacyclie - Platelet inhibitory activity - Eicosaenoic acids
 

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