Thromb Haemost 1985; 53(02): 221-224
DOI: 10.1055/s-0038-1661279
Original Article
Schattauer GmbH Stuttgart

Human Platelet Aggregation and Release Reaction Induced by Platelet Activating Factor (PAF-Acether) – Effects of Acetylsalicylic Acid and External Ionized Calcium

Marco Cattaneo
The Hemophilia and Thrombosis Center “A. Bianchi Bonomi”, University of Milan and Maggiore Hospital, Milan, Italy
,
Maria Teresa Canciani
The Hemophilia and Thrombosis Center “A. Bianchi Bonomi”, University of Milan and Maggiore Hospital, Milan, Italy
,
Pier Mannuccio Mannucci
The Hemophilia and Thrombosis Center “A. Bianchi Bonomi”, University of Milan and Maggiore Hospital, Milan, Italy
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 24. Oktober 1984

Accepted 09. Januar 1985

Publikationsdatum:
18. Juli 2018 (online)

Summary

The effects of the cyclo-oxygenase inhibition on PAF-acether- induced human platelet aggregation and secretion are controversial. We studied the above parameters on citrated platelet-rich plasma of 12 normal subjects before and after the in vivo administration of acetylsalicylic acid (ASA). Individual sensitivities to PAF-acether were highly variable. ASA completely inhibited the platelet secretion induced by low concentrations of PAF-acether, but caused only partial inhibition when platelets were exposed to high concentrations of PAF-acether. The concentration of PAF-acether which overcame the cyclo-oxygenase inhibition varied substantially, depending on the individual sensitivity of the platelets to it. The addition of CaCl2 2 mM to the samples did not affect the extent of the platelet secretion, but increased irreversible aggregation in samples taken both before and after the ASA administration. These data suggest that low concentrations of PAF-acether stimulate the human platelet secretion by activating the cyclo-oxygenase pathway, whereas higher concentrations also trigger other mechanism(s) that suffice to induce human platelet secretion and full aggregation.

 
  • References

  • 1 Benveniste J, Le Couedic JP, Kamoun P. Aggregation of human platelets by platelet activating factor. Lancet 1977; 1: 344-345
  • 2 Cazenave JP, Benveniste J, Mustard JF. Aggregation of rabbit platelets by platelet activating factor is independent of the release reaction and the arachidonate pathway and inhibited by membrane acting drugs. Lab Invest 1979; 41: 275-285
  • 3 McManus LM, Hanahan DJ, Pinkard RN. Human platelet stimulation by Acetyl Glyceryl Ether Phosphorylcholine. J Clin Invest 1981; 67: 903-906
  • 4 Henson PM. Activation of rabbit platelets by platelet activating factor derived by IgE-sensitized basophils. J Clin Invest 1977; 60: 481-490
  • 5 Shaw JO, Printz MP, Hirabayashi K, Henson PM. Role of prostaglandin synthesis in rabbit platelet activation induced by basophil-derived platelet activating factor. J Immunol 1978; 121: 1939-1945
  • 6 Marcus AJ, Safier LB, Ullman HL, Wong KT H, Broekman MJ, Weksler BB, Kaplan KL. Effects of Acetyl Glyceryl Ether Phosphorylcholine on human platelet function in vitro. Blood 1981; 58: 1027-1031
  • 7 Chignard M, Le Couedic JP, Tence M, Vargaftig BB, Benveniste J. The role of platelet activating factor in platelet aggregation. Nature 1979; 279: 799-800
  • 8 Vargaftig BB, Chignard M, Le Couedic JP, Benveniste J. One, two, three or more pathways for platelet aggregation. Acta Med Scand 1980; 642 Suppl 23-29
  • 9 Chesney CM, Pifer DD, Byers LW, Muirhead EE. Effect of platelet activating factor (PAF) on human platelets. Blood 1982; 59: 582-585
  • 10 Kloprogge E, de Haas GH, Gorter G, Akkerman JW N. Stimulus-response coupling in human platelets: evidence against a role of PAF-acether in the third pathway. Thromb Haemostas 1983; 50: 13 (Abstr.)
  • 11 Rao AK, Willis J, Holmsen H, Smith JB. Response to platelet activating factor (PAF) in human platelets is dependent on secreted ADP. Thromb Haemostas 1983; 50: 13 (Abstr.)
  • 12 Clark PO, Hanahan DJ, Pinckard RN. Physical and chemical properties of Platelet Activating Factor obtained from human neutrophils and monocytes and rabbit neutrophils and basophils. Biochim Biophys Acta 1980; 628: 69-75
  • 13 Mustard JF, Perry DW, Kinlough-Rathbone RL, Packham MA. Factors responsible for ADP-induced release reaction of human platelets. Am J Physiol 1975; 228: 1757-1765
  • 14 Glusa E, Markwardt F. Adrenaline-induced reactions of human platelets in hirudin plasma. Haemostasis 1980; 9: 188-192
  • 15 Levin RI, Jaffe EA, Weksler BB, Tack-Goldman K. Nitroglycerine stimulates synthesis of prostacyclin by cultured human endothelial cells. J Clin Invest 1981; 67: 762-769
  • 16 Farr RS, Cox CP, Wardlow ML, Jorgesen R. Preliminary studies of an Acid Labile Factor (ALF) in human sera that inactivates Platelet Activating Factor (PAF). Clin Immunol Immunopathol 1980; 15: 318-330
  • 17 Farr RS, Wardlow ML, Cox CP, Meng KE, Greene DE. Human serum add-labile factor is an acylhydrolase that inactivates plateletactivating factor. Fed Proc 1983; 42: 3120-3122
  • 18 Kinlough-Rathbone RL, Packham MA, Reimers HJ, Cazenave JP, Mustard JF. Mechanisms of platelet shape change, aggregation and release induced by collagen, thrombin or A 23, 187. J Lab Clin Med 1977; 90: 707-719
  • 19 Zucker MB, Peterson J. Inhibition of adenosine diphosphate-induced secondary aggregation and other platelet functions by acetylsalicylic acid ingestion. Proc Soc Exp Biol Med 1968; 127: 547-553