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Thromb Haemost 1984; 52(01): 034-036
DOI: 10.1055/s-0038-1661131
Original Article
Schattauer GmbH Stuttgart

Evaluation of Dose and Route Effects of Platelet Activating Factor-Induced Extravasation in the Guinea Pig

Dean A Handley
The Platelet Section, Sandoz Research Institute, East Hanover, New Jersey, U.S.A.
,
Ronald G Van Valen
The Platelet Section, Sandoz Research Institute, East Hanover, New Jersey, U.S.A.
,
Mary Kay Melden
The Platelet Section, Sandoz Research Institute, East Hanover, New Jersey, U.S.A.
,
Robert N Saunders
The Platelet Section, Sandoz Research Institute, East Hanover, New Jersey, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 14 March 1984

Accepted 05 May 1984

Publication Date:
19 July 2018 (online)

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Summary

Platelet-activating factor (PAF) is a naturally occurring lipid that is reported to induce vessel hyperpermeability leading to loss of protein-rich plasma (extravasation). We have quantitated the systemic extravasation effects of synthetic PAF in the guinea pig by monitoring increases in hematocrit. When given intravenously (10-170 ng/kg), PAF produced dose-dependent increases in hematocrit, with maximal hemoconcentration developing in 5-7 min. In leukopenic animals the expected hematocrit increase was reduced by 57%. PAF given intra-arterially produced the dose-dependent changes in hematocrit similar to the intravenous effects of PAF. However, PAF given intraperitoneally (10-2500 μg/kg) was 800-1100-fold less effective than the other routes and hemoconcentration continued for 30-45 min until a maximal hematocrit was observed. These results show that PAF may markedly influence extravasation of plasma in a dose and route-dependent manner.

Keywords

Platelet-activating factor - Extravasation - Inflammation - Endothelial permeability - Leukocyte
 

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