Pressure-Induced Metabolic Activation of Platelets Demonstrated In Vitro by Determination of Malondialdehyde Formation

Summary

Platelet rich plasma (PRP) exposed in vitro to 200 mm Hg above atmospheric pressure showed a significant increase in malondialdehyde (MDA) formation compared to PRP at atmospheric pressure. This difference is also evident when platelets are incubated with arachidonic acid.

The increase of MDA demonstrates that the increased beta- thromboglobulin and platelet factor 4 in plasma and the shape changes of platelets after pressure stimulation in vitro that were described in a previous paper result from the release reaction.

Pressure-induced effects in vivo are discussed

Keywords

Platelet function - Hypertension - Haemorheology

• References

• 1 Torsellini A, Maggi L, Guidi G, Lombardi V. Modifications of platelet function induced by the variation of hydrostatic pressure in an experimental model. In Haemostasis and Thrombosis. NeriSerneri GG, Prentice CR M. (Eds) Academic Press; London: 1979. pp 375-383
• 2 Torsellini A, Becucci A, Citi S, Cozzolino F, Guidi G, Lombardi V, Vercelli C, Veloci M. Effects of pressure excursions on human platelets. In vitro studies on beta-thromboglobulin and platelet factor 4 release and on platelet sensitivity to ADP aggregation Haematologica 1982; 67: 860-866
• 3 Smith JB, Ingerman CM, Silver MJ. Malondialdehyde formation as an indicator of prostaglandin production by human platelets. J Lab Clin Med 1976; 88: 167-172
• 4 Hamberg M, Samuelsson B. Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets Proc Natl Acad Sci USA 1974; 71: 3400-3404
• 5 Best LC, Jones PB, Russel RG. The relationship between the production of thromboxane B₂ and malondialdehyde by human platelets. Clin Scien 1980; 59: 131-135
• 6 Moncada S, Vane JR. Unstable metabolites of arachidonic acid and their role in haemostasis and thrombosis. Br Med Bull 1978; 34: 129-136
• 7 Botha JH, Leary WP, Asmal AC. Enhanced release of a “prostacyclin-like” substance from aortic strips of spontaneously hypertensive rats. Prostaglandins 1980; 19: 285-289
• 8 Palinski W, Torsellini A, Doni L. Influence of platelet activation on erythrocyte deformability. Thromb Haemostas 1983; 49: 84-86
• 9 Dintenfass L. Hyperviscosity in hypertension. Pergamon Press; (Australia): 1981. p 108
• 10 Dintenfass L, Girolami A. Rigidity of red cells in essential hypertension. Haemostasis 1978; 7: 298-302