Thromb Haemost 1985; 54(04): 799-803
DOI: 10.1055/s-0038-1660136
Original Article
Schattauer GmbH Stuttgart

Early Platelet-Collagen Interactions in Whole Blood and Their Modifications by Aspirin and Dipyridamole Evaluated by a New Method (Basic Wave)

José Luís Pérez-Requejo
1   The Department of Clinical Pathology, Hospital “La Fe”, Valencia, Spain
2   The Research Center, Hospital “La Fe”, Valencia, Spain
,
Justo Aznar
1   The Department of Clinical Pathology, Hospital “La Fe”, Valencia, Spain
,
M Teresa Santos
2   The Research Center, Hospital “La Fe”, Valencia, Spain
,
Juana Vallés
2   The Research Center, Hospital “La Fe”, Valencia, Spain
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 06. Oktober 1984

Accepted 16. September 1985

Publikationsdatum:
26. Juli 2018 (online)

Summary

It is shown that the supernatant of unstirred whole blood at 37° C, stimulated by 1 μg/ml of collagen for 10 sec, produces a rapid generation of pro and antiaggregatory compounds with a final proaggregatory activity which can be detected for more than 60 min on a platelet rich plasma (PRP) by turbidometric aggregometry. A reversible aggregation wave that we have called BASIC wave (for Blood Aggregation Stimulatory and Inhibitory Compounds) is recorded. The collagen stimulation of unstirred PRP produces a similar but smaller BASIC wave. BASIC’s intensity increases if erythrocytes are added to PRP but decreases if white blood cells are added instead. Aspirin abolishes “ex vivo” the ability of whole blood and PRP to generate BASIC waves and dipyridamole “in vitro” significantly reduces BASIC’s intensity in whole blood in every tested sample, but shows little effect in PRP.

 
  • References

  • 1 Packham M. Methods for detection of hypersensitive platelets. Thromb Haemostas 1978; 40: 175-195
  • 2 Bom G VR, Wehmeier A. Inhibition of platelet thrombus formation of chlorpromazine acting to diminish haemolysis. Nature 1979; 282: 212-213
  • 3 Gaarder A, Jonsen J, Laland J, Helme A, Owren PA. Adenosin diphosphate in red cells as a factor in adhesiveness of human blood platelets. Nature 1961; 192: 531-532
  • 4 Subbarao K, Rucinski B, Rausch MA, Schmid K, Niewiarowski S. Binding of dipyridamole to human platelets and to al acid glycoprotein and its significance for the inhibition of adenosine uptake. J Clin Invest 1977; 60: 936-943
  • 5 Willems C, Stel HV, Van Aken WG, Van Mourik JA. Binding and inactivation of prostacyclin (PGI2) by human erythrocytes. Br J Haematol 1983; 54: 43-52
  • 6 Jackubowski JA, Thompson CB, Deykin D. Inactivation of prostacyclin (PGI2) by erythrocytes. Br J Haematol 1983; 54: 658-660
  • 7 Blackwell GJ, Flower RJ, Russell-Smith N, Salmon JA, Thorogood PB, Vane JR. Prostacyclin is produced in whole blood. Br J Pharmacol 1978; 64: 436
  • 8 Weksler BB, Goldstein IM. Prostaglandins: Interactions with platelets and polymorphonuclear leukocytes in hemostasis and inflammation. Am J Med 1980; 68: 419-428
  • 9 Benveniste J, Henson PM, Cochrane CG. Leukocyte dependent histamine release from rabbit platelets: the role of IgE-basophils and platelet activating factor. J Exp Med 1972; 136: 1356-1377
  • 10 Homstra G, Chirt-Hazelhof E, Haddeman E, Ten Hoor F, Nugteren DH. Fish oil feeding lowers thromboxane and prostacyclin production by rat platelets and aorta and does not result in the formation of prostaglandin I3 . Prostaglandins 1981; 21: 727-738
  • 11 Bom G VR. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 1962; 194: 927-929
  • 12 Baumgartner HR. Platelet interaction with collagen fibrils in flowing blood. 1. Reaction of human platelets with a-chymotrypsin-digested subendothelium. Thromb Haemostas 1977; 37: 1-16
  • 13 Cardinal DC, Flower RJ. The electronic aggregometer: a novel device for assessing platelet behaviour in blood. J Pharmacol Methods 1980; 3: 135-158
  • 14 Fox SC, Burgess-Wilson M, Heptinstall S, Mitchell JR A. Platelet aggregation in whole blood determined using the Ultra-Flo 100 platelet counter. Thromb Haemostas 1982; 48: 327-329
  • 15 Saniabadi AR, Lowe G DO, Forbes CD, Prentice CRM, Barbenel JC. Platelet aggregation studies in whole human blood. Thromb Res 1983; 30: 625-632
  • 16 Born G VR, Bergquist D, Arfors KE. Evidence for inhibition of platelet activation in blood by a drug effect on erythrocytes. Nature 1976; 259: 233-235
  • 17 Spitz B, Deckmyn H, Van Assche FA, Vermylen J. Prostacyclin production in whole blood throughout normal pregnancy. Clin Exp Hypert (B) 1983; 2: 191-202
  • 18 Rao AK, Willis J, Holmsen H. A major role of ADP in thromboxane transfer experiments; studies in patients with platelet secretion defects. J Lab Clin Med 1984; 104: 116-126
  • 19 White JG, Gerrard JM. The cell biology of platelets. In: Weissman G. (ed) The Cell Biology of Inflammation. Elsevier; New York: 1980: 83-144
  • 20 Vargaftig BB, Benveniste J. Platelet-activating factor today. Trends Pharmacol Sci 1983; 4: 341-343
  • 21 Homby EJ, Perry CR. 1-0-Hexadecyl-2-Acetyl-SN-3-Glycerophos-phorylcholine (PAF): some effects on the aggregation of human platelets by thrombin or collagen. Thromb Haemostas 1983; 50: 586-587
  • 22 Gresele P, Zoja C, Deckmyn H, Amout J, Vermylen J, Verstraete M. Dipyridamole inhibits platelet aggregation in whole blood. Thromb Haemostas 1983; 50: 852-856
  • 23 Roos H, Pfleger K. Kinetics of adenosin uptake by erythrocytes and influence of dipyridamole. Mol Pharmacol 1972; 8: 417-425
  • 24 Haslam RJ, Lynham JA. Activation and inhibition of blood platelet adenylate cyclase by adenosine or by 2-chloroadenosine. Life Sci 1972; 11: 1143-1154
  • 25 Mills D CB, Smith JB. The influence on platelet aggregation of drugs that effect the accumulation of adenosin 3′:5′-cyclic monophosphate in platelets. Biochem J 1971; 121: 185-196