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Thromb Haemost 1983; 49(01): 018-020
DOI: 10.1055/s-0038-1657306
Original Article
Schattauer GmbH Stuttgart

Increased Urinary β-Thromboglobulin Excretion in Diabetes Assayed with a Modified RIA Kit-Technique

Bernard A van Oost
The Department of Hematology, University Hospital Utrecht, Utrecht, The Netherlands
,
Bernard Veldhuyzen
The Department of Hematology, University Hospital Utrecht, Utrecht, The Netherlands
,
Ans P M Timmermans
The Department of Hematology, University Hospital Utrecht, Utrecht, The Netherlands
,
Jan J Sixma
The Department of Hematology, University Hospital Utrecht, Utrecht, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 06 October 1982

Accepted 06 December 1982

Publication Date:
18 July 2018 (online)

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Summary

A modification of the commercial radioimmunoassay (RIA) kit adapting to the assay of β-thromboglobulin (β-TG) in the urine, is described. The urine was concentrated by dialysis against dry Sepharose G 200. l31I-albumin was used as marker. Experiments in which l25I-β-TG and 131I-albumin were added simultaneously showed a parallel recovery of both markers in the final sample. The sensitivity of the RIA was enhanced by incubation overnight of the urine sample with the antiserum. Increased urinary pTG values were found in 20 patients with diabetes mellitus as compared to 20 young healthy normals. Increased urinary β-TG values correlated with decreased creatinine clearance, but the difference between diabetics and normals was still observed when patients with a creatinine clearance of less than 100 ml/min were excluded. The urine βTG level was correlated with the presence of neovascularization within the diabetic group.

Keywords

β-thromboglobulin - Diabetes - Microangiopathy - Radioimmunoassay
 

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