Thromb Haemost 1982; 48(03): 294-296
DOI: 10.1055/s-0038-1657284
Original Article
Schattauer GmbH Stuttgart

Purification of Human Tissue-Type Plasminogen Activator in Centigram Quantities from Human Melanoma Cell Culture Fluid and Its Conditioning for Use In Vivo

D Collen
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
,
D C Rijken
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
,
J Van Damme
*   Laboratory of Virology, Rega Institute, Department of Human Biology, University of Leuven, Belgium
,
A Billiau
*   Laboratory of Virology, Rega Institute, Department of Human Biology, University of Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Received 18 August 1982

Accepted 13 October 1982

Publication Date:
13 July 2018 (online)

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Summary

Human tissue-type plasminogen activator was produced in centigram quantities from 50–60 1 batches of conditioned medium of a human melanoma cell culture. The yields of the procedure remained essentially unchanged during 50 subsequent preparations. The final products were of high purity as assessed by SDS gel electrophoresis.

These materials, after filtration on 0.22 μM Milliporefilters were sterile and free of viruses and pyrogens. Their stability was very good in the fluid form at temperatures up to 56° C. Material obtained by the present procedure has been used to investigate the biological and thrombolytic properties of tissue-type plasminogen activator.