Correlation Between Noradrenaline Fluxes, Metabolism and Compartmentalisation in Human Platelets

Chantal Legrand; Véronique Dubernard; Philippe Meyer

doi:10.1055/s-0038-1657217

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1982; 48(01): 062-066
DOI: 10.1055/s-0038-1657217

Original Article

Schattauer GmbH Stuttgart

Correlation Between Noradrenaline Fluxes, Metabolism and Compartmentalisation in Human Platelets

Chantal Legrand

^*The Unité de Recherches d'Hémostase et de Thrombose Expérimentale, INSERM U150, Hôpital Lariboisière, Paris, France

,

Véronique Dubernard

^*The Unité de Recherches d'Hémostase et de Thrombose Expérimentale, INSERM U150, Hôpital Lariboisière, Paris, France

,

Philippe Meyer

^**Département de Néphrologie et Département de Pharmacologie, INSERM U7, Hôpital Necker, Paris, France

› Author Affiliations

Abstract

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Summary

(³H) noradrenaline was taken up by human platelets and partially converted into sulfoconjugated noradrenaline. This uptake was inhibited by drugs which have been previously shown to impair the uptake of 5-HT (ouabain, chlorimipramine) or the storage of 5-HT (tyramine, reserpine) by platelets. In addition, tyramine and reserpine stimulated the formation of sulfoconjugated noradrenaline. The efflux of noradrenaline from platelets was measured in parallel and was found to be directly related to the proportion of non metabolized to metabolized noradrenaline in the cells. Unlike tyramine, which induced a similar release of noradrenaline and 5-HT, reserpine was less effective at inducing noradrenaline release than 5-HT release. This study indicates a preferential localization of noradrenaline in the granular pool of human platelets with the existence of an extragranular sulfoconjugated pool which is increased when the granular storage of noradrenaline is impaired. Studies of noradrenaline fluxes and metabolism may be useful in the understanding of both acquired and inherited platelet storage pool defects.

Keywords

Platelets - Noradrenaline - Storage - Metabolism - Fluxes

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References

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