The method of factor VIII purification by chromatography on aminohexyl Sepharose has
been extended so that up to 100 ml of intermediate purity concentrate can be processed
on an 8.6 ml column. The product has a specific activity of 1.8 International Units
of factor VIII per mg of protein. Most of the fibrinogen is removed and antibodies
to blood group substances A and B are not detectable by haemagglutination techniques.
Hepatitis B antigen has been reduced to one sixteenth of that in the starting material,
in preliminary experiments. The process has the added advantage that it concentrates
the factor VIII relative to the starting material.
Keywords
Factor VIII - Hepatitis B - Factor VIII fractionation - Aminoheryl Sepharose
