Summary
Using treatment with thrombin associated with trans-aminomethylcyclohexane carboxylic
acid (t-AMCHA), a simple and rapid method for isolating soluble fibrin complexes (SFC)
from fibrinogen in the plasma was developed. By this procedure, the recovery rates
of SFC and early FDP (mainly X) increased according to the concentration of t-AMCHA,
reaching a maximum at 286 mM t-AMCHA. On the other hand, the recovery rate of fibrinogen
remained below 1.5% and that of late FDP was almost 100% at all concentrations of
t- AMCHA. These results suggested that SFC and FDP could be isolated from fibrinogen
by thrombin and t-AMCHA (286 mM) treatment. Moreover, it was possible to isolate SFC
from FDP using gel filtration after treatment with thrombin and t-AMCHA. The SFC could
be quantified by assay of the eluted fractions containing SFC by the staphylococcal
clumping test.
