Habutobin Splits the Arg¹⁶-Gly¹⁷ Bond in the Aa Chain of Rabbit Fibrinogen

Summary

We reported previously that habutobin, a thrombin-like enzyme from Trimeresurus flavoviridis venom, clotted only rabbit fibrinogen, whereas human, monkey, bovine, dog, rat and guinea-pig fibrinogens were unaffected. In the present study, we investigated the cleavage site of the rabbit Aα chain by habutobin. The fibrinopeptide released by habutobin was identical to the fibrinopeptide A released by thrombin, and its amino acid sequence corresponded to Aα 1-16 of rabbit fibrinogen. It was clarified therefore that habutobin cleaves the Arg¹⁶-Gly¹⁷ bond in the Aa chain of rabbit fibrinogen.

• References

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