Thromb Haemost 1997; 77(05): 0894-0900
DOI: 10.1055/s-0038-1656074
Coagulation
Schattauer GmbH Stuttgart

CD1lb/CD 18 Mediates the Neutrophil Chemotactic Activity of Fibrin Degradation Product D Domain

Thomas J Gross
The Division of Pulmonary, Critical Care, andOccupational Medicine, Department of Internal Medicine University of Iowa College of Medicine Iowa City, IA, USA
,
Keith J Leavell
The Division of Pulmonary, Critical Care, andOccupational Medicine, Department of Internal Medicine University of Iowa College of Medicine Iowa City, IA, USA
,
Michael W Peterson
The Division of Pulmonary, Critical Care, andOccupational Medicine, Department of Internal Medicine University of Iowa College of Medicine Iowa City, IA, USA
› Author Affiliations
Further Information

Publication History

Received 03 September 1996

Accepted after resubmission 07 January 1997

Publication Date:
11 July 2018 (online)

Summary

Coagulation and fibrinolysis universally accompany tissue injury and repair. The accumulation of regionally generated fibrin degradation products (FDP) may modify the local inflammatory response. We have found FDP to be potent neutrophil chemotaxins. We separated plasmin FDP by chromatofocusing and found chemotactic activity limited to fractions containing the fibrinogen D domain (D-D dimer and D monomer). The bioactivity of the D-D dimer did not require an intact cross link site as removal of this sequence with puff adder venom or hypocalcemic plasmic digestion did not decrease chemotaxis. Peptide inhibition studies confirmed that the chemotactic region did not involve terminal gamma chain sequences or alpha chain RGD motifs. The internal gamma chain peptide KYGWTVFQKRLDGSV (PI), known to bind CDllb/CD18, exhibited concentration dependent chemotactic activity. Similarly, monoclonal antibodies directed against CD1lb/CD18 blocked PMN migration to FDP without similar inhibition of chemotaxis to IL-8 or LTB4. Thus, neutrophil chemotaxis to FDP is mediated by interactions between the fibrinogen D domain and CD1lb/CD18.

 
  • References

  • 1 Brown L, Dvorak A, Dvorak H. Leaky vessels, fibrin deposition, and fibrosis: a sequence of events common to solid tumors and to many other types of disease. Am Rev Respir Dis 1989; 140: 1104-1107
  • 2 Ferguson EW, Fretto LJ, McKee PA. A re-examination of the cleavage of fibrinogen and fibrin by plasmin. J Biol Chem 1975; 250: 7210-7218
  • 3 Gaffney PJ. Structure of fibrinogen and degradation products of fibrinogen and fibrin. Br Med Bull 1977; 33: 245-251
  • 4 Ge M, Ryan TJ, Lum H, Malik AB. Fibrinogen degradation product fragment D increases endothelial monolayer permeability. Am J Physiol (Lung Cell Mol Physiol) 1991; 5: L283-L289
  • 5 Hamaguchi M, Morishita Y, Takahashi I, Ogura M, Takamatsu J, Saito H. FDP D-dimer induces the secretion of interleukin-1, urokinase-type plasminogen activator, and plasminogen activator inhibitor type-2 in a human promonocytic leukemia line. Blood 1991; 77: 094-100
  • 6 Robson SC, Shepard EG, Kirsch RE. Fibrin degradation product D-dimer induces the synthesis and release of biologically active IL-lb, IL-6, and plasminogen activator inhibitors from monocytes in vitro. Br J Haematol 1994; 86: 322-326
  • 7 O’Brodovich HM, Weitz JI, Possmayer F. Effect of fibrinogen degradation products and lung ground substance on surfactant function. Biol Neonate 1990; 57: 325-333
  • 8 Leavell KJ, Peterson MW, Gross TJ. The Role of Fibrin Degradation Products in Neutrophil Recruitment to the Lung. Am J Respir Cell Mol Biol 1996; 14: 53-60
  • 9 McKenzie R, Pepper DS, Kay AB. The generation of chemotactic activity for human leukocytes by the action of plasmin on human fibrinogen. Thromb Res 1975; 6: 1-8
  • 10 Metcalf JA, Gallin JI, Nauseef WM, Root RK. Neutrophil Purification. In: Laboratory manual of neutrophil function. Raven Press, NY; NY: 1986: 02-29
  • 11 O’Flaherty JT, Ward PA. Chemotactic Factors and the Neutrophil. Sem. Hematol 1979; 16: 163-174
  • 12 Hynes RO. Integrins: Versatility, modulation, and signaling in cell adhesion. Cell 1992; 69: 11-25
  • 13 Hantgan RR, Endenburg SC, Cavero I, Marguerie G, Uzan A, Sixma JJ, Groot PGd. Inhibition of Platelet Adhesion to Fibrin(ogen) in Flowing whole Blood by Arg-Gly-Asp and Fibrinogen γ-Chain Carboxy Terminal Peptides. Thromb Haemost 1992; 68: 694-700
  • 14 Altieri DC, Duperray A, Plescia J, Thornton GB, Languino LR. Structural recognition of a novel fibrinogen gamma chain sequence by intercellular adhesion molecule-1 mediates leukocyte-endothelium interaction. J Biol Chem 1995; 270: 696-699
  • 15 Altieri DC, Agbanyo FR, Plescia J, Ginsberg MH, Edgington TS, Plow EF. A unique recognition site mediates the interaction of fibrinogen with the leukocyte integrin Mac-1 (CD1 lb/CD 18). J Biol Chem 1990; 265: 12119-12122
  • 16 Altieri DC, Plescia J, Plow EF. The structural motif glycine 190-valine 202 of the fibrinogen γ chain interacts with CD1 lb/CD 18 integrin (αmβ2, Mac-1) and promotes leukocyte adhesion. J Biol Chem 1993; 268: 1847-1853
  • 17 Tang L, Ugarova TP, Plow EF, Eaton JW. Molecular determinants of acute inflammatory responses to biomaterials. J Clin Invest 1996; 97: 1329-1334
  • 18 Deutsch DG, Mertz ET. Plasminogen: Purification from human plasma by affinity chromatography. Science 1970; 170: 1095-1096
  • 19 Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254
  • 20 Kalvaria I, Corrigall AV, Kirsch RE. Separation and purification of rat fibrinogen degradation products D1 and E by chromatofocusing. Thromb Res 1983; 29: 459-464
  • 21 Kalvaria I, Rabinowitz S, Frith L, Kirsch RE. Fibrinogen synthesis in the rat: Role of the C terminal end of the gamma chain of fragment D1. Thromb Res 1986; 43: 287-291
  • 22 Robson SC, Saunders R, Purves LR, Jager Cd, Corrigall A, Kirsch RE. Fibrin and fibrinogen degradation products with an intact D-domain C-terminal γ chain inhibit an early step in accessory cell-dependent lymphocyte mitogenesis. Blood 1993; 81: 3006-3014
  • 23 Moskowitz KA, Budzynski AZ. The DD(E) complex is maintained by a composite fibrin polymerization site. Biochem 1994; 33: 12937-12944
  • 24 Gaffney PJ, Tymkewycz PM. Fibrinogen/Fibrin degradation products: Relevance of unique structures to the study of thrombosis. In: Fibrinogen:Biochemistry, biological functions, and gene regulation. Mosesson WM. ed. Elsevier Science Publishers; Amsterdam: 1988. 3. 285-295
  • 25 Purves L, Purves M, Brandt W. Cleavage of fibrin-derived D-dimer into monomers by endopeptidase from puff adder venom acting at cross-linked sites of the gamma chain. Biochem 1987; 26: 4640-4646
  • 26 Purves L, Naidoo D. Zinc binding by fibrin facilitates proteolysis by a snake (puff adder) venom protease. Sem Thromb Hemo 1992; 18: 252-255
  • 27 Lindsey GG, Brown G, Purves LR. Calcium binding to human fibrinogen – localization of two calcium specific sites. Thromb Res 1978; 13: 345-350
  • 28 Purves L, Lindsey GG, Brown G, Franks J. Stabilization of the plasmin digestion products of fibrinogen and fibrin by calcium ions. Thromb Res 1978; 12: 473-484
  • 29 Nieuwenhuizen W, Vermond A, Haverkate F. Factors influencing the structure of terminal plasmin degradation products of human fibrinogen and fibrin. Biochim Biophys 1981; 667: 321-327
  • 30 Moyle M, Foster DL, McGrath DE, Brown SM, Laroche Y, Meutter JD, Stanssens P, Bogowitz CA, Fried VA, Ely JA, Soule HR, Vlasuk GP. A hookworm glycoprotein that inhibits neutrophil function is a ligand of the integrin CDllb/CD18. J Biol Chem 1994; 269: 10008-10015
  • 31 Senior RM, Gresham HD, Griffin GL, Brown EJ, Chung AE. Entactin stimulates neutrophil adhesion and chemotaxis through interactions between its Arg-Gly-Asp (RGD) domain and the leukocyte response integrin. J Clin Invest 1992; 90: 2251-2257
  • 32 Loike JD, Sodeik B, Cao L, Leucona S, Weitz JI, Detmers PA, Wright SD, Silverstein SC. CD 11 c/CD 18 on neutrophils recognizes a domain at the N terminus of the Aa chain of fibrinogen. Proc Natl Acad Sci USA 1991; 88: 1044-1048
  • 33 Shainoff JR, Steams DJ, DiBello PM, Hishikawa-Itoh Y. Characterization of a mode of specific binding of fibrin monomer through its amino-terminal domain macrophages and macrophage cell-lines. Thromb Haemo 1990; 63: 193-203
  • 34 Henkin J, Marcotte P, Yang H. The plasminogen-plasmin system. Prog Cardiovasc Dis 1991; 34: 135-164
  • 35 Vassalli J, Sappino A, Belin D. The plasminogen activator/plasmin system. J Clin Invest 1991; 88: 604-619
  • 36 Idell S, James KK, Levin EG, Schwartz BS, Manchanda N, Maunder RJ, Martin TR, McLarty J, Fair DS. Local abnormalities in coagulation and fibrinolytic pathways predispose to alveolar fibrin deposition in the adult respiratory distress syndrome. J Clin Invest 1989; 84: 695-705
  • 37 Perez RL, Duncan A, Hunter RL, GW Jr S. Elevated D dimer in the lungs and blood of patients with sarcoidosis. Chest 1993; 103: 1100-1106
  • 38 Nakstad B, Lyberg T, Skjonsberg OH, Boye NP. Local activation of the coagulation and fibrinolysis systems in lung disease. Thromb Res 1990; 56: 827-838
  • 39 Hart DA. Regulation of plasminogen activators in connective tissues: Potential for thrombolytic therapy in collagen vascular diseases. J Rheum 1989; 16: 1184-1191
  • 40 Thompson WD, Harvey JA, Kazmi MA, Stout AJ. Fibrinolysis and angio-genesis in wound healing. J Pathol 1991; 165: 311-318
  • 41 Dang CH, Bell WR, Kaiser D, Wong A. Disorganization of cultured vascular endothelial monolayers by fibrin fragment D. Science 1985; 227: 1487-1490
  • 42 Doolittle RF, Watt KWK, Cottrell BA, Strong DD, Riley M. The amino acid sequence of the α-chain of human fibrinogen. Nature 1979; 280: 464-468
  • 43 Diamond MS, Aguilar JG, Bickford JK, Corbi AL, Springer TA. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CDllb/CD18) for four distinct adhesion ligands. J Cell Biol 1993; 120: 1031-1043
  • 44 Zhou L, Lee DHS, Plescia J, Lau CY, Altieri DC. Differential ligand binding domain specificities of recombinant CD1 lb/CD 18 integrin I-domain. J Biol Chem 1994; 269: 17075-17079
  • 45 Chapman H, Bertozzi P, Reilly J. Role of enzymes mediating thrombosis and thrombolysis in lung disease. Chest 1988; 93: 1256-1263
  • 46 Bach-Gansmo E, Halvorsen S, Godal H, Skjonsberg O. D-dimers are degraded by human neutrophil elastase. Thromb Res 1996; 82: 177-186
  • 47 Plow EF. The major fibrinolytic proteases of human leukocytes. Biochim Biophys Acta 1980; 630: 47-56
  • 48 Gross TJ, Cobb SM, Peterson MW. Asbestos exposure increases para-cellular transport of fibrin degradation products across human airway epithelium. Am J Physiol (Lung Cell Mol Physiol) 1994; 266: L287-L295