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Thromb Haemost 1997; 77(02): 394-398
DOI: 10.1055/s-0038-1655974
Original Article
Schattauer GmbH Stuttgart

C5a Induces Tissue Factor Activity on Endothelial Cells

Kei Ikeda
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Kohei Nagasawa
2   The Department of Internal Medicine, Saga Medical School, Saga, Japan
,
Takahiko Horiuchi
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Tomomi Tsuru
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Hiroaki Nishizaka
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Yoshiyuki Niho
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
› Author Affiliations
Further Information

Publication History

Received 30 April 1996

Accepted after revision 24 October 1996

Publication Date:
10 July 2018 (online)

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Summary

Tissue factor (TF) is an integral membrane glycoprotein that serves as a cofactor for blood coagulation factor VIIa. The induction of TF on the surface of endothelial cells is initiated by various stimuli including lipopolysaccharide, interleukin-1β, and tumor necrosis factor α. We have demonstrated that recombinant human C5a induces TF activity in a dose-dependent fashion in human umbilical vein endothelial cells (HUVEC). Peak activity (4.9-fold increase) was obtained 3-6 h after treatment with 10 μM C5a. TF mRNA as assessed by RT-PCR method was also significantly increased (3.75-fold) after 3 h incubation with C5a, suggesting that C5a induces TF activity on HUVEC, at least in part, by enhancing the level of TF mRNA. The increase in TF activity by C5a was inhibited by methylprednisolone. The induction of TF on endothelial cells by C5a may represent one of many potential interrelationships between the inflammatory and coagulation schemes.

 

  • References

  • 1 Nemerson Y. Tissue factor and hemostasis. Blood 1988; 71: 01-08
    PubMedGoogle Scholar
  • 2 Moore KL, Andreoli SP, Esmon NL, Esmon CT, Bang NU. Endotoxin enhances tissue factor and suppresses thrombomodulin expression of human vascular endothelium in vitro. J Clin Invest 1987; 79: 124-130
    CrossrefPubMedGoogle Scholar
  • 3 Crossman DC, Carr DP, Tuddenham EG, Pearson JD, McVey JH. The regulation of tissue factor mRNA in human endothelial cells in response to endotoxin or phorbol ester. J Biol Chem 1990; 265: 9782-9287
    PubMedGoogle Scholar
  • 4 Nawroth PP, Stern DM. Modulation of endothelial cell hemostatic properties by tumor necrosis factor. J Exp Med 1986; 163: 740-745
    CrossrefPubMedGoogle Scholar
  • 5 Tijburg PN, Ryan J, Stem DM, Wollitzky B, Rimon S, Rimon A, Handley D, Nawroth P, Sixma JJ, de GrootPG. Activation of the coagulation mechanism on tumor necrosis factor-stimulated cultured endothelial cells and their extracellular matrix. The role of flow and factor IX/IXa. J Biol Chem 1991; 266: 12067-12074
    PubMedGoogle Scholar
  • 6 Bevilacqua MP, Pober JS, Majeau GR, Cotran RS, Gimbrone MAJ. Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells. J Exp Med 1984; 160: 618-623
    CrossrefPubMedGoogle Scholar
  • 7 Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MAJ. Interleukin-1 activation of vascular endothelium. Effects on procoagulant activity and leukocyte adhesion. Am J Pathol 1985; 121: 394-403
    PubMedGoogle Scholar
  • 8 Taylor FBJ, Chang A, Esmon CT, D’Angelo A, Vigano-D’Angelo S, Blick KE. Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon. J Clin Invest 1987; 79: 918-925
    CrossrefPubMedGoogle Scholar
  • 9 Taylor FBJ, Emerson TEJ, Jordan R, Chang AK, Blick KE. Antithrom bin-III prevents the lethal effects of Escherichia coli infusion in baboons. Circ Shock 1988; 26: 227-235
    PubMedGoogle Scholar
  • 10 Taylor FBJ, Chang A, Ruf W, Morrissey JH, Hinshaw L, Catlett R, Blick K, Edgington TS, Lethal E. coli septic shock is prevented by blocking tissue factor with monoclonal antibody. Circ Shock 1991; 33: 127-134
    PubMedGoogle Scholar
  • 11 Haviland DL, McCoy RL, Whitehead WT, Akama H, Molmenti EP, Brown A, Haviland JC, Parks WC, Perlmutter DH, Wetsel RA. Cellular Expression of the C5a Anaphylatoxin Receptor (C5aR):Demonstration of C5aR on Nonmyeloid Cells of the Liver and Lung. J Immunol 1995; 154: 1861-1869
    PubMedGoogle Scholar
  • 12 Murphy HS, Shayman JA, Till GO, Mahrougui M, Owens CB, Ryan US, Ward PA. Superoxide responses of endothelial cells to C5a and TNF-alpha:divergent signal transduction pathways. Am J Physiol 1992; 263: L51-L59
    PubMedGoogle Scholar
  • 13 Foreman KE, Vaporciyan AA, Bonish BK, Jones ML, Johnson KJ, Glovsky MM, Eddy SM, Ward PA. C5a-induced expression of P-selectin in endothelial cells. J Clin Invest 1994; 94: 1147-1155
    CrossrefPubMedGoogle Scholar
  • 14 Muhlfelder TW, Niemetz J, Kreutzer D, Beebe D, Ward PA, Rosenfeld SI. C5 chemotactic fragment induces leukocyte production of tissue factor activity:a link between complement and coagulation. J Clin Invest 1979; 63: 147-150
    CrossrefPubMedGoogle Scholar
  • 15 Sitrin RG, Kaltreider HB, Ansfield MJ, Webster RO. Procoagulant activity of rabbit alveolar macrophages. Am Rev Respir Dis 1983; 128: 282-287
    PubMedGoogle Scholar
  • 16 Janco RL, Morris P. Serum augments the generation of monocyte procoagulant stimulated by bacterial lipopolysaccharide or chemotactic fragments of C5. Thromb Res 1983; 32: 73-86
    CrossrefPubMedGoogle Scholar
  • 17 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-2756
    CrossrefPubMedGoogle Scholar
  • 18 Surprenant YM, Zuckerman SH. A novel microtiter plate assay for the quantitation of procoagulant activity on adherent monocytes, macrophage and endothelial cells. Thromb Res 1989; 53: 339-346
    CrossrefPubMedGoogle Scholar
  • 19 Bolhuis PA, Sylva-Steenland RM, Tutuar EA, van derHelm HJ. Comparison of the spectrophotometric determination and the two-stage coagulation assay of tissue factor activity. Thromb Res 1982; 27: 429-434
    CrossrefPubMedGoogle Scholar
  • 20 Faucette KJ, Fitzgerald LA, Liu L, Parker CJ, Rodgers GM. Characterization of the interactions between procoagulant albumin and human endothelial cells. Blood 1993; 82: 2684-2692
    PubMedGoogle Scholar
  • 21 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-159
    PubMedGoogle Scholar
  • 22 Potgens AJG, Lubsen NH, van AltenaG, Schoenmakers JGG, Ruiter DJ, de WaalRMW. Measurement of tissue factor messenger RNA levels in human endothelial cells by a quantitative RT-PCR assay. Thromb Haemost 1994; 71: 208-213
    PubMedGoogle Scholar
  • 23 Horiuchi T, Hatta N, Matsumoto M, Ohtsuka H, Collins FS, Kobayashi Y, Fujita S. Nonsense mutations at Arg-1947 in two cases of familial neurofibromatosis type 1 in Japanese. Hum Genet 1994; 93: 81-83
    PubMedGoogle Scholar
  • 24 Scarpati EM, Wen D, Broze GJJ, Miletich JP, Flandermeyer RR, Siegel NR, Sadler JE. Human tissue factor:cDNA sequence and chromosome localization of the gene. Biochemistry 1987; 26: 5234-5238
    CrossrefPubMedGoogle Scholar
  • 25 Carre PC, Mortenson RL, King Jr. T, Noble PW, Sable CL, Riches DW. Increased expression of the interleukin-8 gene by alveolar macrophages in idiopathic pulmonary fibrosis. A potential mechanism for the recruitment and activation of neutrophils in lung fibrosis. J Clin Invest 1991; 88: 1802-1810
    CrossrefPubMedGoogle Scholar
  • 26 Colucci M, Balconi G, Lorenzet R, Pietra A, Locati D, Donati MB, Semeraro N. Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 1983; 71: 1893-1896
    CrossrefPubMedGoogle Scholar
  • 27 Goodman MG, Chenoweth DE, Weigle WO. Potentiation of the primary humoral immune response in vitro by C5a anaphylatoxin. J Immunol 1982; 129: 70-75
    PubMedGoogle Scholar
  • 28 Morgan EL, Thoman ML, Te WWH. Anaphylatoxin-mediated regulation of the immune response. II. C5a-mediated enhancement of human humoral and T cell-mediated immune responses. J Immunol 1983; 130: 1257-1261
    PubMedGoogle Scholar
  • 29 Montz H, Fuhrmann A, Schulze M, Gotze O. Regulation of the human autologous T cell proliferation by endogenously generated C5a. Cell Immunol 1990; 127: 337-351
    CrossrefPubMedGoogle Scholar
  • 30 Hogan MM, Yancey KB, Vogel SN. Role of C5a in the induction of tumor-icidal activity in C3H/HeJ (Lpsd) and C3H/OuJ (Lpsn) macrophages. J Leukocyte Biol 1989; 46: 565-570
    CrossrefPubMedGoogle Scholar
  • 31 Okusawa S, Dinarello CA, Yancey KB, Endres S, Lawley TJ, Frank MM, Burke JF, Gelfand JA. C5a induction of human interleukin 1. Synergistic effect with endotoxin or interferon-gamma. J Immunol 1987; 139: 2635-2640
    PubMedGoogle Scholar
  • 32 Cavaillon JM, Fitting C, Haeffner-Cavaillon N. Recombinant C5a enhances interleukin 1 and tumor necrosis factor release by lipopolysaceha-ride-stimulated monocytes and macrophages. Eur J Immunol 1990; 20: 253-257
    CrossrefPubMedGoogle Scholar
  • 33 Schindler R, Gelfand JA, Dinarello CA. Recombinant C5a stimulates transcription rather than translation of interleukin-1 (IL-1) and tumor necrosis factor:translational signal provided by lipopolysaccharide or IL-1 itself. Blood 1990; 76: 1631-1638
    PubMedGoogle Scholar
  • 34 Scholz W, McClurg MR, Cardenas GJ, Smith M, Noonan DJ, Hugli TE, Morgan EL. C5a-mediated release of interleukin 6 by human monocytes. Clin Immunol Immunopathol 1990; 57: 297-307
    CrossrefPubMedGoogle Scholar
  • 35 Morgan EL, Noonan DJ, Weigle WO, Hugli TE. Identification and characterization of the effector region within human C5a responsible for stimulation of IL-6 synthesis. J Immunol 1992; 148: 3937-3942
    PubMedGoogle Scholar
  • 36 Ember JA, Sanderson SD, Hugli TE, Morgan EL. Induction of interleukin-8 synthesis from monocytes by human C5a anaphylatoxin. Am J Pathol 1994; 144: 393-403
    PubMedGoogle Scholar
  • 37 Bevilacqua MP, Pober JS, Majeau GR, Fiers W, Cotran RS, Gimbrone Jr M. Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium:characterization and comparison with the actions of interleukin 1. Proc Natl Acad Sci USA 1986; 83: 4533-4537
    CrossrefPubMedGoogle Scholar
  • 38 Ruggeri ZM, Zimmerman TS. von Willebrand factor and von Willebrand disease. Blood 1987; 70: 895-904
    PubMedGoogle Scholar
  • 39 Bottles KD, Morrissey JH. Dexamethasone enhances agonist induction of tissue factor in monocytes but not in endothelial cells. Blood Coagul Fibrinolysis 1993; 04: 405-414
    CrossrefPubMedGoogle Scholar
  • 40 Latour JG, Leger C. Prevention by glucocorticoids of disseminated intraascular coagulation induced by endotoxin:mechanisms. J Lab Clin Med 1975; 85: 934-949
    PubMedGoogle Scholar
  • 41 Yoshikawa T, Murakami M, Furukawa Y, Takemura S, Kondo M. Prevention by methylprednisolone of disseminated intravascular coagulation induced by sustained infusion of endotoxin in rats. Haemostasis 1983; 13: 268-273
    PubMedGoogle Scholar