The Effect of the Contact Factors of the Coagulation System on Platelet Aggregation^[*]

Summary

In these investigations it was shown that the contact factors of the coagulation system - isolated as in the contact activation test according to Egli or as Celite eluate (Waaler) - do not influence platelet aggregation. Platelet aggregation was measured by the rotation method of Wright.

It was concluded that contact of blood with wettable surfaces leads to activation of the coagulation system and independently to aggregation of platelets. By heating of plasma to 56° a platelet aggregating principle is liberated; the significance of this finding needs further investigation.

^* Supported by Deutsche Forschungsgemeinschaft.

• References

• 1 Gastaldi P. A, Larrieu M. J, Caen J. Availability of platelet factor 3 and activation of factor XII in thrombasthenia. Nature (Lond.) 207: 422 1965;
• 2 Deyken D, Pritzker C. R, Scolnick E. M. Plasma co-factors in adenosine diphosphate -induced aggregation of human platelets. Nature (Lond.) 208: 286 1965;
• 3 Egli H, Buscha H. Zur Differenzierung und Kalziumabhängigkeit von Kontaktaktivierung benetzbarer Oberflächen. Thrombos. Diathes. haemorrh. (Stuttg.) 3: 604 1959;
• 4 Feissly R, Lüdin H. Microscopie par contacts de phase. Rev. Hémat 4: 481 1949;
• 5 Glynn M. F, Movat H. Z, Murphy E. A, Mustard J. F. Study of platelet adhesiveness and aggregation with latex particles. J. Lab. clin. Med 65: 179 1965;
• 6 Hardisty R. M, Hutton R. A. The Kaolin clotting time of platelet rich plasma; A test of platelet factor 3 availability. Brit. J. Haemat 11: 258 1965;
• 7 Horowitz H. I, Fujimo M. M. Association of factors XI and XII with blood platelets. Proc. Soc. exp. Biol. (N. Y.) 119: 487 1965;
• 8 Hugues J. Accolement des plaquettes aux structures conjunctives périvasculaires. Thrombos. Diathes. haemorrh. (Stuttg.) 8: 241 1962;
• 9 Hugues J, Lapiere G. Nouvelles recherches sur l’accolement des plaquettes aux fibres des collagènes Thrombos. Diathes. haemorrh. (Stuttg.) 11: 327 1964;
• 10 Iatridis P. G, Ferguson D. H. The plasmatic atmosphere of blood platelets. Evidence that only fibrinogen, AcG, and activated Hageman factor are present on the surface of platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 13: 114 1965;
• 11 Jürgens J. The significance of the Hagemann factor for the effect of wetable surface on thrombocytes. Thrombos. Diathes. haemorrh. (Stuttg.) 7: 48 1962;
• 12 Niewiarowski St, Bankowski E, Rogowicka J. Studies on the adsorption and activation of the Hageman factor (F. XII) by collagen and elastin. Thrombos. Diathes. haemorrh. (Stuttg.) 14: 387 1965;
• 13 Nossel H. L. The contact phase of blood coagulation. Oxford: 1964
• 14 Sharp A. A. Viscous metamorphosis of blood platelets. A study of the relationship to coagulation factors and fibrin formation. Brit. J. Haemat 4: 28 1958;
• 15 Spaet T. H, Cintron J. Studies on platelet factor 3 availability. Brit. J. Haemat 11: 269 1965;
• 16 Spaet T. H. International symposium on thrombosis and embolism 29.8.—1.9. 1965. Basle; Switzerland:
• 17 Waaler B. A. Contact activation in the intrinsic blood clotting system. Scand. J. clin. Invest. 9 Suppl. 37 1959
• 18 Wright H. P. The adhesiveness of blood platelets in normal subjects with varying concentration of anticoagulants. J. Path. Bact 53: 255 1942;
• 19 Zucker M. B, Borelli J. Changes in platelet ADP concentration and phosphate distribution during viscous metamorphosis and clot retraction. In “Blood Platelets” Henry Ford Hospital symposium, 1960, Boston, Mass