Fibrinolytic Activity of Tissue Sections of the Dog Kidney

J Prokopowicz; L Rejniak; S Niewiarowski; K Worowski

doi:10.1055/s-0038-1655554

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1964; 12(02): 396-406
DOI: 10.1055/s-0038-1655554

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Fibrinolytic Activity of Tissue Sections of the Dog Kidney^[*]

Authors

J Prokopowicz

¹Department of Physiological Chemistry. Head: Doc. Dr. S. Niewiarowski M.D. and Department of Pathological Anatomy, Head: Prof. Dr. L. Komczyúski, M. D., Medical School, Bialystok, Poland
L Rejniak

¹Department of Physiological Chemistry. Head: Doc. Dr. S. Niewiarowski M.D. and Department of Pathological Anatomy, Head: Prof. Dr. L. Komczyúski, M. D., Medical School, Bialystok, Poland
S Niewiarowski

¹Department of Physiological Chemistry. Head: Doc. Dr. S. Niewiarowski M.D. and Department of Pathological Anatomy, Head: Prof. Dr. L. Komczyúski, M. D., Medical School, Bialystok, Poland
K Worowski

¹Department of Physiological Chemistry. Head: Doc. Dr. S. Niewiarowski M.D. and Department of Pathological Anatomy, Head: Prof. Dr. L. Komczyúski, M. D., Medical School, Bialystok, Poland

Abstract

Summary

A method for estimating fibrinolytic activity of the tissue sections has been worked out. This method consists in covering tissue sections with thin fibrin layers and in microscopic evaluation of their digestion. Using two types of fibrin layers: a) prepared from fibrinogen containing plasminogen, b) prepared from plasminogen free fibrinogen, it is possible to differentiate, whether the digestion is due to the plasminogen activator or to the active enzyme (plasmin).

Fibrinolytic activity of the section of dog kidney tissue has been investigated. Experiments were performed on normal kidneys and on kidneys damaged by the mercury chloride intoxication. Studying normal dog kidney it has been found that plasminogen activator is present in juxtaglomerular cells, macula densa and Goormaghtigh cells. It appears that the endothelial cells of the glomerular vessels contain, besides plasminogen activator, an active enzyme, probably plasmin. In the medullary portion of the section of normal kidney tissue no fibrinolytic activity has been found.

In dogs intoxicated with mercury chloride the plasminogen activator decreases in vascular endothelia but it completely disappears in the juxtaglomerular apparatus. The injury of the juxtaglomerular apparatus due to mercury chloride intoxication has been also found by routine histopatho-logical investigations.

It is suggested that the plasminogen activator, secreted by the kidney into the general circulation, orginates mainly in the juxtaglomerular cells.

Full Text

References

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Fibrinolytic Activity of Tissue Sections of the Dog Kidney[*]

Authors

Fibrinolytic Activity of Tissue Sections of the Dog Kidney^[*]